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Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex

Fekete, Veronika ; Cierna, Maria ; Poláková, Silvia LU ; Piskur, Jure LU and Sulo, Pavol (2007) In FEMS Yeast Research 7(8). p.1237-1247
Abstract
Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages... (More)
Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ethidium, Saccharomyces/Kluyveromyces complex, petite mutation, mtDNA, bromide, mitochondria
in
FEMS Yeast Research
volume
7
issue
8
pages
1237 - 1247
publisher
Oxford University Press
external identifiers
  • wos:000250298000004
  • scopus:35448951798
ISSN
1567-1364
DOI
10.1111/j.1567-1364.2007.00287.x
language
English
LU publication?
yes
id
fa59bda7-43f9-49a0-a935-4edbf760fd95 (old id 653143)
date added to LUP
2016-04-01 11:52:52
date last changed
2022-02-25 22:40:49
@article{fa59bda7-43f9-49a0-a935-4edbf760fd95,
  abstract     = {{Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species.}},
  author       = {{Fekete, Veronika and Cierna, Maria and Poláková, Silvia and Piskur, Jure and Sulo, Pavol}},
  issn         = {{1567-1364}},
  keywords     = {{ethidium; Saccharomyces/Kluyveromyces complex; petite mutation; mtDNA; bromide; mitochondria}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1237--1247}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Yeast Research}},
  title        = {{Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex}},
  url          = {{http://dx.doi.org/10.1111/j.1567-1364.2007.00287.x}},
  doi          = {{10.1111/j.1567-1364.2007.00287.x}},
  volume       = {{7}},
  year         = {{2007}},
}