Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Deep eutectic solvents for the preservation of concentrated proteins : the case of lysozyme in 1 : 2 choline chloride : glycerol

Sanchez-Fernandez, Adrian LU orcid ; Prevost, Sylvain and Wahlgren, Marie LU orcid (2022) In Green Chemistry 24(11). p.4437-4442
Abstract

The stability and activity of a model protein, lysozyme, in 1 : 2 choline chloride : glycerol was characterised across a wide range of protein concentrations, i.e., between 4 and 143 mg ml−1, where the protein is soluble and physically stable. Lysozyme folds into a globular conformation in this deep eutectic solvent like that in the native state, but with subtle variations in its internal structure. The protein remained stable even after 40 days of storage at room temperature and retrieved its native conformation and activity upon rehydration. These results show the potential of deep eutectic solvents as sustainable, non-toxic, and synthetically accessible media for the storage and preservation of proteins in a concentrated... (More)

The stability and activity of a model protein, lysozyme, in 1 : 2 choline chloride : glycerol was characterised across a wide range of protein concentrations, i.e., between 4 and 143 mg ml−1, where the protein is soluble and physically stable. Lysozyme folds into a globular conformation in this deep eutectic solvent like that in the native state, but with subtle variations in its internal structure. The protein remained stable even after 40 days of storage at room temperature and retrieved its native conformation and activity upon rehydration. These results show the potential of deep eutectic solvents as sustainable, non-toxic, and synthetically accessible media for the storage and preservation of proteins in a concentrated regime without the requirement of other excipients.

(Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Green Chemistry
volume
24
issue
11
pages
6 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85131261500
ISSN
1463-9262
DOI
10.1039/d1gc04378a
language
English
LU publication?
yes
id
fa72eb9c-c8a5-4910-a6d2-1bb8ff850ff5
date added to LUP
2022-08-18 14:52:03
date last changed
2023-12-18 11:13:02
@article{fa72eb9c-c8a5-4910-a6d2-1bb8ff850ff5,
  abstract     = {{<p>The stability and activity of a model protein, lysozyme, in 1 : 2 choline chloride : glycerol was characterised across a wide range of protein concentrations, i.e., between 4 and 143 mg ml<sup>−1</sup>, where the protein is soluble and physically stable. Lysozyme folds into a globular conformation in this deep eutectic solvent like that in the native state, but with subtle variations in its internal structure. The protein remained stable even after 40 days of storage at room temperature and retrieved its native conformation and activity upon rehydration. These results show the potential of deep eutectic solvents as sustainable, non-toxic, and synthetically accessible media for the storage and preservation of proteins in a concentrated regime without the requirement of other excipients.</p>}},
  author       = {{Sanchez-Fernandez, Adrian and Prevost, Sylvain and Wahlgren, Marie}},
  issn         = {{1463-9262}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{11}},
  pages        = {{4437--4442}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Green Chemistry}},
  title        = {{Deep eutectic solvents for the preservation of concentrated proteins : the case of lysozyme in 1 : 2 choline chloride : glycerol}},
  url          = {{http://dx.doi.org/10.1039/d1gc04378a}},
  doi          = {{10.1039/d1gc04378a}},
  volume       = {{24}},
  year         = {{2022}},
}