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The immune cell atlas of human neuroblastoma

Verhoeven, Bronte Manouk ; Mei, Shenglin ; Olsen, Thale Kristin ; Gustafsson, Karin ; Valind, Anders LU orcid ; Lindström, Axel ; Gisselsson, David LU ; Fard, Shahrzad Shirazi ; Hagerling, Catharina LU and Kharchenko, Peter V. , et al. (2022) In Cell Reports Medicine 3(6).
Abstract

Understanding the complete immune cell composition of human neuroblastoma (NB) is crucial for the development of immunotherapeutics. Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. We provide a comprehensive immune cell landscape and characterize cell-state changes from normal tissue to NB. Our analysis reveals 27 immune cell subtypes, including distinct subpopulations of myeloid, NK, B, and T cells. Several different cell types demonstrate a survival benefit. In contrast to adult cancers and previous NB studies, we show an increase in inflammatory monocyte cell state when contrasting normal... (More)

Understanding the complete immune cell composition of human neuroblastoma (NB) is crucial for the development of immunotherapeutics. Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. We provide a comprehensive immune cell landscape and characterize cell-state changes from normal tissue to NB. Our analysis reveals 27 immune cell subtypes, including distinct subpopulations of myeloid, NK, B, and T cells. Several different cell types demonstrate a survival benefit. In contrast to adult cancers and previous NB studies, we show an increase in inflammatory monocyte cell state when contrasting normal and tumor tissue, while no differences in cytotoxicity and exhaustion score for T cells, nor in Treg activity, are observed. Our receptor-ligand interaction analysis reveals a highly complex interactive network of the NB microenvironment from which we highlight several interactions that we suggest for future therapeutic studies.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cancer, human, immune cell landscape, immuno-oncology, immunotherapy, neuroblastoma, pediatric cancer, prognosis, single-cell RNA sequencing, survival
in
Cell Reports Medicine
volume
3
issue
6
article number
100657
publisher
Elsevier
external identifiers
  • pmid:35688160
  • scopus:85133101304
ISSN
2666-3791
DOI
10.1016/j.xcrm.2022.100657
language
English
LU publication?
yes
id
face2277-a77e-4736-8bee-38c5151134a1
date added to LUP
2022-10-06 15:45:48
date last changed
2024-06-15 00:11:30
@article{face2277-a77e-4736-8bee-38c5151134a1,
  abstract     = {{<p>Understanding the complete immune cell composition of human neuroblastoma (NB) is crucial for the development of immunotherapeutics. Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. We provide a comprehensive immune cell landscape and characterize cell-state changes from normal tissue to NB. Our analysis reveals 27 immune cell subtypes, including distinct subpopulations of myeloid, NK, B, and T cells. Several different cell types demonstrate a survival benefit. In contrast to adult cancers and previous NB studies, we show an increase in inflammatory monocyte cell state when contrasting normal and tumor tissue, while no differences in cytotoxicity and exhaustion score for T cells, nor in Treg activity, are observed. Our receptor-ligand interaction analysis reveals a highly complex interactive network of the NB microenvironment from which we highlight several interactions that we suggest for future therapeutic studies.</p>}},
  author       = {{Verhoeven, Bronte Manouk and Mei, Shenglin and Olsen, Thale Kristin and Gustafsson, Karin and Valind, Anders and Lindström, Axel and Gisselsson, David and Fard, Shahrzad Shirazi and Hagerling, Catharina and Kharchenko, Peter V. and Kogner, Per and Johnsen, John Inge and Baryawno, Ninib}},
  issn         = {{2666-3791}},
  keywords     = {{cancer; human; immune cell landscape; immuno-oncology; immunotherapy; neuroblastoma; pediatric cancer; prognosis; single-cell RNA sequencing; survival}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{6}},
  publisher    = {{Elsevier}},
  series       = {{Cell Reports Medicine}},
  title        = {{The immune cell atlas of human neuroblastoma}},
  url          = {{http://dx.doi.org/10.1016/j.xcrm.2022.100657}},
  doi          = {{10.1016/j.xcrm.2022.100657}},
  volume       = {{3}},
  year         = {{2022}},
}