Supported fluid lipid bilayer as a scaffold to direct assembly of RNA nanostructures
(2017) In Methods in Molecular Biology 1632. p.107-122- Abstract
RNA architectonics offers the possibility to design and assemble RNA into specific shapes, such as nanoscale 3D solids or nanogrids. Combining the minute size of these programmable shapes with precise positioning on a surface further enhances their potential as building blocks in nanotechnology and nanomedicine. Here we describe a bottom-up approach to direct the arrangement of nucleic acid nanostructures by using a supported fluid lipid bilayer as a surface scaffold. The strong attractive electrostatic interactions between RNA polyanions and cationic lipids promote RNA adsorption and self-assembly. Protocol steps for the characterization of assembled RNA complexes via several complementary methods (QCM-D, ellipsometry, confocal... (More)
RNA architectonics offers the possibility to design and assemble RNA into specific shapes, such as nanoscale 3D solids or nanogrids. Combining the minute size of these programmable shapes with precise positioning on a surface further enhances their potential as building blocks in nanotechnology and nanomedicine. Here we describe a bottom-up approach to direct the arrangement of nucleic acid nanostructures by using a supported fluid lipid bilayer as a surface scaffold. The strong attractive electrostatic interactions between RNA polyanions and cationic lipids promote RNA adsorption and self-assembly. Protocol steps for the characterization of assembled RNA complexes via several complementary methods (QCM-D, ellipsometry, confocal fluorescence microscopy, AFM) are also provided. Due to their tunable nature, lipid bilayers can be used to organize RNA laterally on the micrometer scale and thus facilitate the building of more complex 3D structures. The bilayer-based approach can be extended to other programmable RNA or DNA objects to construct intricate structures, such as 2D grids or 3D cages, with high precision.
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- author
- Dabkowska, Aleksandra P. LU ; Michanek, Agnes LU ; Jaeger, Luc ; Chworos, Arkadiusz ; Nylander, Tommy LU and Sparr, Emma LU
- organization
- publishing date
- 2017
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- 2D ordering, AFM, Confocal fluorescence microscopy, Ellipsometry, Nanostructure, QCM-D, RNA architectonics, Supported lipid bilayer
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 1632
- pages
- 16 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:28730435
- scopus:85029371935
- ISSN
- 10643745
- DOI
- 10.1007/978-1-4939-7138-1_7
- language
- English
- LU publication?
- yes
- id
- fb35a29c-1917-4913-b860-062d1dade66c
- date added to LUP
- 2017-10-05 07:44:44
- date last changed
- 2024-03-17 21:57:57
@inbook{fb35a29c-1917-4913-b860-062d1dade66c,
abstract = {{<p>RNA architectonics offers the possibility to design and assemble RNA into specific shapes, such as nanoscale 3D solids or nanogrids. Combining the minute size of these programmable shapes with precise positioning on a surface further enhances their potential as building blocks in nanotechnology and nanomedicine. Here we describe a bottom-up approach to direct the arrangement of nucleic acid nanostructures by using a supported fluid lipid bilayer as a surface scaffold. The strong attractive electrostatic interactions between RNA polyanions and cationic lipids promote RNA adsorption and self-assembly. Protocol steps for the characterization of assembled RNA complexes via several complementary methods (QCM-D, ellipsometry, confocal fluorescence microscopy, AFM) are also provided. Due to their tunable nature, lipid bilayers can be used to organize RNA laterally on the micrometer scale and thus facilitate the building of more complex 3D structures. The bilayer-based approach can be extended to other programmable RNA or DNA objects to construct intricate structures, such as 2D grids or 3D cages, with high precision.</p>}},
author = {{Dabkowska, Aleksandra P. and Michanek, Agnes and Jaeger, Luc and Chworos, Arkadiusz and Nylander, Tommy and Sparr, Emma}},
booktitle = {{Methods in Molecular Biology}},
issn = {{10643745}},
keywords = {{2D ordering; AFM; Confocal fluorescence microscopy; Ellipsometry; Nanostructure; QCM-D; RNA architectonics; Supported lipid bilayer}},
language = {{eng}},
pages = {{107--122}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{Supported fluid lipid bilayer as a scaffold to direct assembly of RNA nanostructures}},
url = {{http://dx.doi.org/10.1007/978-1-4939-7138-1_7}},
doi = {{10.1007/978-1-4939-7138-1_7}},
volume = {{1632}},
year = {{2017}},
}