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Supported fluid lipid bilayer as a scaffold to direct assembly of RNA nanostructures

Dabkowska, Aleksandra P. LU ; Michanek, Agnes LU ; Jaeger, Luc; Chworos, Arkadiusz; Nylander, Tommy LU and Sparr, Emma LU (2017) In Methods in Molecular Biology 1632. p.107-122
Abstract

RNA architectonics offers the possibility to design and assemble RNA into specific shapes, such as nanoscale 3D solids or nanogrids. Combining the minute size of these programmable shapes with precise positioning on a surface further enhances their potential as building blocks in nanotechnology and nanomedicine. Here we describe a bottom-up approach to direct the arrangement of nucleic acid nanostructures by using a supported fluid lipid bilayer as a surface scaffold. The strong attractive electrostatic interactions between RNA polyanions and cationic lipids promote RNA adsorption and self-assembly. Protocol steps for the characterization of assembled RNA complexes via several complementary methods (QCM-D, ellipsometry, confocal... (More)

RNA architectonics offers the possibility to design and assemble RNA into specific shapes, such as nanoscale 3D solids or nanogrids. Combining the minute size of these programmable shapes with precise positioning on a surface further enhances their potential as building blocks in nanotechnology and nanomedicine. Here we describe a bottom-up approach to direct the arrangement of nucleic acid nanostructures by using a supported fluid lipid bilayer as a surface scaffold. The strong attractive electrostatic interactions between RNA polyanions and cationic lipids promote RNA adsorption and self-assembly. Protocol steps for the characterization of assembled RNA complexes via several complementary methods (QCM-D, ellipsometry, confocal fluorescence microscopy, AFM) are also provided. Due to their tunable nature, lipid bilayers can be used to organize RNA laterally on the micrometer scale and thus facilitate the building of more complex 3D structures. The bilayer-based approach can be extended to other programmable RNA or DNA objects to construct intricate structures, such as 2D grids or 3D cages, with high precision.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
2D ordering, AFM, Confocal fluorescence microscopy, Ellipsometry, Nanostructure, QCM-D, RNA architectonics, Supported lipid bilayer
in
Methods in Molecular Biology
volume
1632
pages
16 pages
publisher
Humana Press
external identifiers
  • scopus:85029371935
ISSN
10643745
DOI
10.1007/978-1-4939-7138-1_7
language
English
LU publication?
yes
id
fb35a29c-1917-4913-b860-062d1dade66c
date added to LUP
2017-10-05 07:44:44
date last changed
2018-01-07 12:20:42
@inbook{fb35a29c-1917-4913-b860-062d1dade66c,
  abstract     = {<p>RNA architectonics offers the possibility to design and assemble RNA into specific shapes, such as nanoscale 3D solids or nanogrids. Combining the minute size of these programmable shapes with precise positioning on a surface further enhances their potential as building blocks in nanotechnology and nanomedicine. Here we describe a bottom-up approach to direct the arrangement of nucleic acid nanostructures by using a supported fluid lipid bilayer as a surface scaffold. The strong attractive electrostatic interactions between RNA polyanions and cationic lipids promote RNA adsorption and self-assembly. Protocol steps for the characterization of assembled RNA complexes via several complementary methods (QCM-D, ellipsometry, confocal fluorescence microscopy, AFM) are also provided. Due to their tunable nature, lipid bilayers can be used to organize RNA laterally on the micrometer scale and thus facilitate the building of more complex 3D structures. The bilayer-based approach can be extended to other programmable RNA or DNA objects to construct intricate structures, such as 2D grids or 3D cages, with high precision.</p>},
  author       = {Dabkowska, Aleksandra P. and Michanek, Agnes and Jaeger, Luc and Chworos, Arkadiusz and Nylander, Tommy and Sparr, Emma},
  issn         = {10643745},
  keyword      = {2D ordering,AFM,Confocal fluorescence microscopy,Ellipsometry,Nanostructure,QCM-D,RNA architectonics,Supported lipid bilayer},
  language     = {eng},
  pages        = {107--122},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Supported fluid lipid bilayer as a scaffold to direct assembly of RNA nanostructures},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7138-1_7},
  volume       = {1632},
  year         = {2017},
}