Continuous lipase-catalyzed production of wax ester using silicone tubing
Wehtje, E. LU ; Costes, D. LU and Adlercreutz, P. LU
(1999)
In JAOCS, Journal of the American Oil Chemists' Society
76(12).
p.1489-1493
- Abstract
Enzymatic synthesis of cetyl palmitate was performed in a solvent-free system at 65 °C using immobilized Candida antarctica lipase. Batch reactions at controlled water activity showed that the yield could be increased from 88.8 to 99.1% by decreasing the water activity from 1 to 0.05. A continuous reactor configuration was constructed, where two tubular reactors were run in sequence with a separation container in between, in which the water phase was separated from the wax ester phase. The reactor was run for 1 wk at low flow rate (0.005 g/min) with very good operational stability and a productivity of 7.2 g d-1 using 0.4 g of biocatalyst. The activity of the individual preparations decreased during operation. The first... (More)
Enzymatic synthesis of cetyl palmitate was performed in a solvent-free system at 65 °C using immobilized Candida antarctica lipase. Batch reactions at controlled water activity showed that the yield could be increased from 88.8 to 99.1% by decreasing the water activity from 1 to 0.05. A continuous reactor configuration was constructed, where two tubular reactors were run in sequence with a separation container in between, in which the water phase was separated from the wax ester phase. The reactor was run for 1 wk at low flow rate (0.005 g/min) with very good operational stability and a productivity of 7.2 g d-1 using 0.4 g of biocatalyst. The activity of the individual preparations decreased during operation. The first reactor had only 30% activity left after 1 wk of operation whereas the second reactor showed only a 10% decrease. This difference in enzyme stability is a direct result of the different water activity in the two reactors.
(Less)
- author
- Wehtje, E.
LU
; Costes, D.
LU
and Adlercreutz, P.
LU
- organization
- publishing date
- 1999-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- JAOCS, Journal of the American Oil Chemists' Society
- volume
- 76
- issue
- 12
- pages
- 5 pages
- publisher
- The American Oil Chemists' Society
- external identifiers
-
- scopus:0033352125
- ISSN
- 0003-021X
- DOI
- 10.1007/s11746-999-0190-4
- language
- English
- LU publication?
- yes
- id
- fbb343bb-b125-4a5c-ac08-086c07434892
- date added to LUP
- 2019-06-20 16:01:01
- date last changed
- 2022-01-31 22:13:24
@article{fbb343bb-b125-4a5c-ac08-086c07434892,
abstract = {{<p>Enzymatic synthesis of cetyl palmitate was performed in a solvent-free system at 65 °C using immobilized Candida antarctica lipase. Batch reactions at controlled water activity showed that the yield could be increased from 88.8 to 99.1% by decreasing the water activity from 1 to 0.05. A continuous reactor configuration was constructed, where two tubular reactors were run in sequence with a separation container in between, in which the water phase was separated from the wax ester phase. The reactor was run for 1 wk at low flow rate (0.005 g/min) with very good operational stability and a productivity of 7.2 g d<sup>-1</sup> using 0.4 g of biocatalyst. The activity of the individual preparations decreased during operation. The first reactor had only 30% activity left after 1 wk of operation whereas the second reactor showed only a 10% decrease. This difference in enzyme stability is a direct result of the different water activity in the two reactors.</p>}},
author = {{Wehtje, E. and Costes, D. and Adlercreutz, P.}},
issn = {{0003-021X}},
language = {{eng}},
month = {{12}},
number = {{12}},
pages = {{1489--1493}},
publisher = {{The American Oil Chemists' Society}},
series = {{JAOCS, Journal of the American Oil Chemists' Society}},
title = {{Continuous lipase-catalyzed production of wax ester using silicone tubing}},
url = {{http://dx.doi.org/10.1007/s11746-999-0190-4}},
doi = {{10.1007/s11746-999-0190-4}},
volume = {{76}},
year = {{1999}},
}