Advanced

Human fibroblasts lacking trans-stilbene oxide active glutathione transferase exhibit increased cell death when exposed to polycyclic aromatic hydrocarbons

Pessah-Rasmussen, H LU ; Stavenow, L.; Seidegård, J LU and Berglund, Anna (1992) In Pharmacology and Toxicology1987-01-01+01:002004-01-01+01:00 70(5 Pt 1). p.5-361
Abstract

Glutathione transferases (GST) are detoxifying enzymes who act with many endogenous and exogenous substances such as polycyclic aromatic hydrocarbons (PAH). The GST activity towards trans-stilbene oxide (GST-tSBO) is inherited in an autosomal dominant fashion and can be separated in high (GST-positive) and low (GST-negative) phenotypes when measured in blood. Human fibroblast cultures were established from males matched for age, smoking habits and clinical manifestations of atherosclerosis. Matched pairs of GST-negative and GST-positive fibroblasts were studied. There was a very strong correlation between the levels of GST-tSBO in peripheral blood and in cultured fibroblasts within the same individual. When fibroblasts were exposed to... (More)

Glutathione transferases (GST) are detoxifying enzymes who act with many endogenous and exogenous substances such as polycyclic aromatic hydrocarbons (PAH). The GST activity towards trans-stilbene oxide (GST-tSBO) is inherited in an autosomal dominant fashion and can be separated in high (GST-positive) and low (GST-negative) phenotypes when measured in blood. Human fibroblast cultures were established from males matched for age, smoking habits and clinical manifestations of atherosclerosis. Matched pairs of GST-negative and GST-positive fibroblasts were studied. There was a very strong correlation between the levels of GST-tSBO in peripheral blood and in cultured fibroblasts within the same individual. When fibroblasts were exposed to benzo(a)pyrene (BP) or dimethylbenzanthracene (DMBA) GST-negative cells produced relatively more collagen than GST-positive cells. GST-negative fibroblasts showed a greater cell death than GST-positive fibroblasts as well among controls as after exposure to PAH. It is concluded that lack of GST-tSBO is easily discriminated in cultured skin fibroblasts. GST-negative and GST-positive fibroblasts showed different susceptibility towards some toxic stimuli that might be of importance in atherogenesis.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aged, Cell Death, Cells, Cultured, Collagen, DNA, Fibroblasts, Glutathione Transferase, Humans, Male, Middle Aged, Polycyclic Compounds, Skin, Stilbenes, Journal Article, Research Support, Non-U.S. Gov't
in
Pharmacology and Toxicology1987-01-01+01:002004-01-01+01:00
volume
70
issue
5 Pt 1
pages
5 - 361
publisher
Wiley-Blackwell
external identifiers
  • scopus:0026538563
ISSN
0901-9928
DOI
language
English
LU publication?
yes
id
fc292e60-e29d-427c-a41f-32af0f2003ba
date added to LUP
2017-08-11 15:57:25
date last changed
2018-05-29 10:34:14
@article{fc292e60-e29d-427c-a41f-32af0f2003ba,
  abstract     = {<p>Glutathione transferases (GST) are detoxifying enzymes who act with many endogenous and exogenous substances such as polycyclic aromatic hydrocarbons (PAH). The GST activity towards trans-stilbene oxide (GST-tSBO) is inherited in an autosomal dominant fashion and can be separated in high (GST-positive) and low (GST-negative) phenotypes when measured in blood. Human fibroblast cultures were established from males matched for age, smoking habits and clinical manifestations of atherosclerosis. Matched pairs of GST-negative and GST-positive fibroblasts were studied. There was a very strong correlation between the levels of GST-tSBO in peripheral blood and in cultured fibroblasts within the same individual. When fibroblasts were exposed to benzo(a)pyrene (BP) or dimethylbenzanthracene (DMBA) GST-negative cells produced relatively more collagen than GST-positive cells. GST-negative fibroblasts showed a greater cell death than GST-positive fibroblasts as well among controls as after exposure to PAH. It is concluded that lack of GST-tSBO is easily discriminated in cultured skin fibroblasts. GST-negative and GST-positive fibroblasts showed different susceptibility towards some toxic stimuli that might be of importance in atherogenesis.</p>},
  author       = {Pessah-Rasmussen, H and Stavenow, L. and Seidegård, J and Berglund, Anna},
  issn         = {0901-9928},
  keyword      = {Aged,Cell Death,Cells, Cultured,Collagen,DNA,Fibroblasts,Glutathione Transferase,Humans,Male,Middle Aged,Polycyclic Compounds,Skin,Stilbenes,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {5 Pt 1},
  pages        = {5--361},
  publisher    = {Wiley-Blackwell},
  series       = {Pharmacology and Toxicology1987-01-01+01:002004-01-01+01:00},
  title        = {Human fibroblasts lacking trans-stilbene oxide active glutathione transferase exhibit increased cell death when exposed to polycyclic aromatic hydrocarbons},
  url          = {http://dx.doi.org/},
  volume       = {70},
  year         = {1992},
}