Differential expression of cytochrome c oxidase subunit III gene in castes of the termite Reticulitermes santonensis
Liénard, Marjorie LU
; Lassance, Jean-Marc
LU
; Paulmier, I
; Picimbon, Jean-Francois
LU
and Löfstedt, Christer
LU
(2006)
In Journal of Insect Physiology
52(6).
p.551-557
- Abstract
- Social insects such as termites live in colonies in which cooperation is assumed by all individuals developing into castes to which specific tasks are allocated. Little has been reported about molecular aspects underlying termite caste-specific gene expression. Genetic regulation has recently been hypothesized to govern caste-specific traits and physiology in social insects. Cytochrome c oxidase (COX) has been shown to be an interesting candidate for expression study in insects. We used the cytochrome c oxidase subunit III gene (COXIII) that was cloned from mRNA in a lower termite, Reticulitermes santonensis De Feytaud (Isoptera; Rhinotermitidae). The full-length cDNA encodes a protein of 262 amino acids that shows high degree of homology... (More)
- Social insects such as termites live in colonies in which cooperation is assumed by all individuals developing into castes to which specific tasks are allocated. Little has been reported about molecular aspects underlying termite caste-specific gene expression. Genetic regulation has recently been hypothesized to govern caste-specific traits and physiology in social insects. Cytochrome c oxidase (COX) has been shown to be an interesting candidate for expression study in insects. We used the cytochrome c oxidase subunit III gene (COXIII) that was cloned from mRNA in a lower termite, Reticulitermes santonensis De Feytaud (Isoptera; Rhinotermitidae). The full-length cDNA encodes a protein of 262 amino acids that shows high degree of homology with other insects COXIIIs. Reverse transcriptase-PCR and real-time PCR were performed to compare gene expression between larvae, workers, nymphs and soldiers. Analyses performed on head cDNAs revealed that COXIII is differentially expressed between castes. The level of COXIII is caste-regulated with an increase in workers (similar to 1.9-fold) and nymphs (similar to 2.8-fold) and a decrease in soldiers (0.8-fold) compared to the expression level in larvae (1.0-fold). These results may emphasize the physiological importance of COX in the termite brain at different developmental stages. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/159708
- author
- Liénard, Marjorie
LU
; Lassance, Jean-Marc
LU
; Paulmier, I
; Picimbon, Jean-Francois
LU
and Löfstedt, Christer
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Insect Physiology
- volume
- 52
- issue
- 6
- pages
- 551 - 557
- publisher
- Elsevier
- external identifiers
-
- pmid:16545394
- wos:000238556200002
- scopus:33646769636
- ISSN
- 1879-1611
- DOI
- 10.1016/j.jinsphys.2006.02.002
- language
- English
- LU publication?
- yes
- id
- fc478ce2-f41e-4edb-8b72-0b5780a64827 (old id 159708)
- date added to LUP
- 2016-04-01 16:15:01
- date last changed
- 2022-01-28 18:23:06
@article{fc478ce2-f41e-4edb-8b72-0b5780a64827,
abstract = {{Social insects such as termites live in colonies in which cooperation is assumed by all individuals developing into castes to which specific tasks are allocated. Little has been reported about molecular aspects underlying termite caste-specific gene expression. Genetic regulation has recently been hypothesized to govern caste-specific traits and physiology in social insects. Cytochrome c oxidase (COX) has been shown to be an interesting candidate for expression study in insects. We used the cytochrome c oxidase subunit III gene (COXIII) that was cloned from mRNA in a lower termite, Reticulitermes santonensis De Feytaud (Isoptera; Rhinotermitidae). The full-length cDNA encodes a protein of 262 amino acids that shows high degree of homology with other insects COXIIIs. Reverse transcriptase-PCR and real-time PCR were performed to compare gene expression between larvae, workers, nymphs and soldiers. Analyses performed on head cDNAs revealed that COXIII is differentially expressed between castes. The level of COXIII is caste-regulated with an increase in workers (similar to 1.9-fold) and nymphs (similar to 2.8-fold) and a decrease in soldiers (0.8-fold) compared to the expression level in larvae (1.0-fold). These results may emphasize the physiological importance of COX in the termite brain at different developmental stages.}},
author = {{Liénard, Marjorie and Lassance, Jean-Marc and Paulmier, I and Picimbon, Jean-Francois and Löfstedt, Christer}},
issn = {{1879-1611}},
language = {{eng}},
number = {{6}},
pages = {{551--557}},
publisher = {{Elsevier}},
series = {{Journal of Insect Physiology}},
title = {{Differential expression of cytochrome c oxidase subunit III gene in castes of the termite <i>Reticulitermes santonensis</i>}},
url = {{http://dx.doi.org/10.1016/j.jinsphys.2006.02.002}},
doi = {{10.1016/j.jinsphys.2006.02.002}},
volume = {{52}},
year = {{2006}},
}