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Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection

Jerre, Elsa; Bungum, Mona LU ; Evenson, Donald and Giwercman, Aleksander LU (2019) In Fertility and Sterility
Abstract

Objective: To determine whether high DNA stainability (HDS), as assessed by the sperm chromatin structure assay (SCSA), predicts the risk of early miscarriage after in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI). Design: Retrospective cohort study of consecutive pregnancies after IVF and ICSI treatment. Setting: Reproductive medicine center. Patient(s): A total of 1,602 pregnancies after 832 IVF and 770 ICSI treatments. Intervention(s): HDS measured using SCSA. Main Outcome Measure(s): Early miscarriage (≤12 weeks). Result(s): The HDS represents the proportion of immature spermatozoa lacking the normal exchange of histone for protamine-complexed DNA, and the outcome parameter was early miscarriage (≤12 weeks).... (More)

Objective: To determine whether high DNA stainability (HDS), as assessed by the sperm chromatin structure assay (SCSA), predicts the risk of early miscarriage after in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI). Design: Retrospective cohort study of consecutive pregnancies after IVF and ICSI treatment. Setting: Reproductive medicine center. Patient(s): A total of 1,602 pregnancies after 832 IVF and 770 ICSI treatments. Intervention(s): HDS measured using SCSA. Main Outcome Measure(s): Early miscarriage (≤12 weeks). Result(s): The HDS represents the proportion of immature spermatozoa lacking the normal exchange of histone for protamine-complexed DNA, and the outcome parameter was early miscarriage (≤12 weeks). For all treatments, the odds ratio (OR)and 95% confidence interval (CI)for early miscarriage was 1.41 (1.07–1.85)if HDS >15% compared with HDS ≤15%. When comparing the two HDS categories, for ICSI, the OR was 1.44 (1.01–2.04)whereas for IVF the results were not statistically significant. Conclusion(s): There is a small but increased risk of early miscarriage if HDS >15% compared with HDS ≤15%. This increased risk is seen only after ICSI, not after IVF. These findings suggest that HDS can be used as a predictor of an increased risk of miscarriage in ICSI treatments.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
DNA fragmentation, early miscarriage rate, high DNA stainability, in vitro fertilization, sperm DNA integrity
in
Fertility and Sterility
publisher
Elsevier
external identifiers
  • scopus:85064767329
ISSN
0015-0282
DOI
10.1016/j.fertnstert.2019.03.013
language
English
LU publication?
yes
id
fc5f77c5-a62e-45dc-8382-bd344794b05c
date added to LUP
2019-05-06 13:40:20
date last changed
2019-08-14 04:36:03
@article{fc5f77c5-a62e-45dc-8382-bd344794b05c,
  abstract     = {<p>Objective: To determine whether high DNA stainability (HDS), as assessed by the sperm chromatin structure assay (SCSA), predicts the risk of early miscarriage after in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI). Design: Retrospective cohort study of consecutive pregnancies after IVF and ICSI treatment. Setting: Reproductive medicine center. Patient(s): A total of 1,602 pregnancies after 832 IVF and 770 ICSI treatments. Intervention(s): HDS measured using SCSA. Main Outcome Measure(s): Early miscarriage (≤12 weeks). Result(s): The HDS represents the proportion of immature spermatozoa lacking the normal exchange of histone for protamine-complexed DNA, and the outcome parameter was early miscarriage (≤12 weeks). For all treatments, the odds ratio (OR)and 95% confidence interval (CI)for early miscarriage was 1.41 (1.07–1.85)if HDS &gt;15% compared with HDS ≤15%. When comparing the two HDS categories, for ICSI, the OR was 1.44 (1.01–2.04)whereas for IVF the results were not statistically significant. Conclusion(s): There is a small but increased risk of early miscarriage if HDS &gt;15% compared with HDS ≤15%. This increased risk is seen only after ICSI, not after IVF. These findings suggest that HDS can be used as a predictor of an increased risk of miscarriage in ICSI treatments.</p>},
  author       = {Jerre, Elsa and Bungum, Mona and Evenson, Donald and Giwercman, Aleksander},
  issn         = {0015-0282},
  keyword      = {DNA fragmentation,early miscarriage rate,high DNA stainability,in vitro fertilization,sperm DNA integrity},
  language     = {eng},
  month        = {04},
  publisher    = {Elsevier},
  series       = {Fertility and Sterility},
  title        = {Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection},
  url          = {http://dx.doi.org/10.1016/j.fertnstert.2019.03.013},
  year         = {2019},
}