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Coevolution with bacteria drives the evolution of aerobic fermentation in Lachancea kluyveri

Zhou, Nerve LU ; Swamy, Krishna B S; Leu, Jun Yi; McDonald, Michael J.; Galafassi, Silvia LU ; Compagno, Concetta and Piskur, Jure LU (2017) In PLoS ONE 12(3).
Abstract

The Crabtree positive yeasts, such as Saccharomyces cerevisiae, prefer fermentation to respiration, even under fully aerobic conditions. The selective pressures that drove the evolution of this trait remain controversial because of the low ATP yield of fermentation compared to respiration. Here we propagate experimental populations of the weak-Crabtree yeast Lachancea kluyveri, in competitive co-culture with bacteria. We find that L. kluyveri adapts by producing quantities of ethanol lethal to bacteria and evolves several of the defining characteristics of Crabtree positive yeasts. We use precise quantitative analysis to show that the rate advantage of fermentation over aerobic respiration is insufficient to provide an overall growth... (More)

The Crabtree positive yeasts, such as Saccharomyces cerevisiae, prefer fermentation to respiration, even under fully aerobic conditions. The selective pressures that drove the evolution of this trait remain controversial because of the low ATP yield of fermentation compared to respiration. Here we propagate experimental populations of the weak-Crabtree yeast Lachancea kluyveri, in competitive co-culture with bacteria. We find that L. kluyveri adapts by producing quantities of ethanol lethal to bacteria and evolves several of the defining characteristics of Crabtree positive yeasts. We use precise quantitative analysis to show that the rate advantage of fermentation over aerobic respiration is insufficient to provide an overall growth advantage. Thus, the rapid consumption of glucose and the utilization of ethanol are essential for the success of the aerobic fermentation strategy. These results corroborate that selection derived from competition with bacteria could have provided the impetus for the evolution of the Crabtree positive trait.

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publication status
published
subject
in
PLoS ONE
volume
12
issue
3
publisher
Public Library of Science
external identifiers
  • scopus:85015338789
  • wos:000396091800029
ISSN
1932-6203
DOI
10.1371/journal.pone.0173318
language
English
LU publication?
yes
id
fe65a9a6-2abc-446e-bb7d-0d85db0a8f26
date added to LUP
2017-04-12 08:01:27
date last changed
2018-05-06 04:32:28
@article{fe65a9a6-2abc-446e-bb7d-0d85db0a8f26,
  abstract     = {<p>The Crabtree positive yeasts, such as Saccharomyces cerevisiae, prefer fermentation to respiration, even under fully aerobic conditions. The selective pressures that drove the evolution of this trait remain controversial because of the low ATP yield of fermentation compared to respiration. Here we propagate experimental populations of the weak-Crabtree yeast Lachancea kluyveri, in competitive co-culture with bacteria. We find that L. kluyveri adapts by producing quantities of ethanol lethal to bacteria and evolves several of the defining characteristics of Crabtree positive yeasts. We use precise quantitative analysis to show that the rate advantage of fermentation over aerobic respiration is insufficient to provide an overall growth advantage. Thus, the rapid consumption of glucose and the utilization of ethanol are essential for the success of the aerobic fermentation strategy. These results corroborate that selection derived from competition with bacteria could have provided the impetus for the evolution of the Crabtree positive trait.</p>},
  articleno    = {e0173318},
  author       = {Zhou, Nerve and Swamy, Krishna B S and Leu, Jun Yi and McDonald, Michael J. and Galafassi, Silvia and Compagno, Concetta and Piskur, Jure},
  issn         = {1932-6203},
  language     = {eng},
  month        = {03},
  number       = {3},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Coevolution with bacteria drives the evolution of aerobic fermentation in Lachancea kluyveri},
  url          = {http://dx.doi.org/10.1371/journal.pone.0173318},
  volume       = {12},
  year         = {2017},
}