Taurine and N-acetylcysteine treatments prevent memory impairment and metabolite profile alterations in the hippocampus of high-fat diet-fed female mice
Garcia-Serrano, Alba M ; Vieira, João LU ; Fleischhart, Veronika and Duarte, Joao LU
(2023)
In Nutritional Neuroscience
26(11).
p.1090-1102
- Abstract
- Background: Obesity constitutes a risk factor for cognitive impairment. In rodent models, long-term exposure to obesogenic diets leads to hippocampal taurine accumulation. Since taurine has putative cyto-protective effects, hippocampal taurine accumulation in obese and diabetic models might constitute a counteracting response to metabolic stress. Objective: We tested the hypothesis that treatment with taurine or with N-acetylcysteine (NAC), which provides cysteine for the synthesis of taurine and glutathione, prevent high-fat diet (HFD)-associated hippocampal alterations and memory impairment. Methods: Female mice were fed either a regular diet or HFD. Some mice had access to 3%(w/v) taurine or 3%(w/v) NAC in the drinking water. After 2... (More)
- Background: Obesity constitutes a risk factor for cognitive impairment. In rodent models, long-term exposure to obesogenic diets leads to hippocampal taurine accumulation. Since taurine has putative cyto-protective effects, hippocampal taurine accumulation in obese and diabetic models might constitute a counteracting response to metabolic stress. Objective: We tested the hypothesis that treatment with taurine or with N-acetylcysteine (NAC), which provides cysteine for the synthesis of taurine and glutathione, prevent high-fat diet (HFD)-associated hippocampal alterations and memory impairment. Methods: Female mice were fed either a regular diet or HFD. Some mice had access to 3%(w/v) taurine or 3%(w/v) NAC in the drinking water. After 2 months, magnetic resonance spectroscopy (MRS) was used to measure metabolite profiles. Memory was assessed in novel object and novel location recognition tests. Results: HFD feeding caused memory impairment in both tests, and reduced concentration of lactate, phosphocreatine-to-creatine ratio, and the neuronal marker N-acetylaspartate in the hippocampus. Taurine and NAC prevented HFD-induced memory impairment and N-acetylaspartate reduction. NAC, but not taurine, prevented the reduction of lactate and phosphocreatine-to-creatine ratio. MRS revealed NAC/taurine-induced increase of hippocampal glutamate and GABA levels. Conclusion: NAC and taurine can prevent memory impairment, while only NAC prevents alterations of metabolite concentrations in HFD-exposed female mice. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/ff29b6b6-d0ac-4e6d-9066-d7330fa0c820
- author
- Garcia-Serrano, Alba M
; Vieira, João
LU
; Fleischhart, Veronika
and Duarte, Joao
LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Obesity, diabetes, MRS, metabolism, neurochemical profile, lactate, N-acetylaspartate (NAA), neuroprotection
- in
- Nutritional Neuroscience
- volume
- 26
- issue
- 11
- pages
- 1090 - 1102
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:36222315
- scopus:85139980203
- ISSN
- 1476-8305
- DOI
- 10.1080/1028415X.2022.2131062
- language
- English
- LU publication?
- yes
- id
- ff29b6b6-d0ac-4e6d-9066-d7330fa0c820
- date added to LUP
- 2022-12-05 21:31:40
- date last changed
- 2023-12-03 13:37:31
@article{ff29b6b6-d0ac-4e6d-9066-d7330fa0c820,
abstract = {{Background: Obesity constitutes a risk factor for cognitive impairment. In rodent models, long-term exposure to obesogenic diets leads to hippocampal taurine accumulation. Since taurine has putative cyto-protective effects, hippocampal taurine accumulation in obese and diabetic models might constitute a counteracting response to metabolic stress. Objective: We tested the hypothesis that treatment with taurine or with N-acetylcysteine (NAC), which provides cysteine for the synthesis of taurine and glutathione, prevent high-fat diet (HFD)-associated hippocampal alterations and memory impairment. Methods: Female mice were fed either a regular diet or HFD. Some mice had access to 3%(w/v) taurine or 3%(w/v) NAC in the drinking water. After 2 months, magnetic resonance spectroscopy (MRS) was used to measure metabolite profiles. Memory was assessed in novel object and novel location recognition tests. Results: HFD feeding caused memory impairment in both tests, and reduced concentration of lactate, phosphocreatine-to-creatine ratio, and the neuronal marker N-acetylaspartate in the hippocampus. Taurine and NAC prevented HFD-induced memory impairment and N-acetylaspartate reduction. NAC, but not taurine, prevented the reduction of lactate and phosphocreatine-to-creatine ratio. MRS revealed NAC/taurine-induced increase of hippocampal glutamate and GABA levels. Conclusion: NAC and taurine can prevent memory impairment, while only NAC prevents alterations of metabolite concentrations in HFD-exposed female mice.}},
author = {{Garcia-Serrano, Alba M and Vieira, João and Fleischhart, Veronika and Duarte, Joao}},
issn = {{1476-8305}},
keywords = {{Obesity; diabetes; MRS; metabolism; neurochemical profile; lactate; N-acetylaspartate (NAA); neuroprotection}},
language = {{eng}},
number = {{11}},
pages = {{1090--1102}},
publisher = {{Taylor & Francis}},
series = {{Nutritional Neuroscience}},
title = {{Taurine and N-acetylcysteine treatments prevent memory impairment and metabolite profile alterations in the hippocampus of high-fat diet-fed female mice}},
url = {{http://dx.doi.org/10.1080/1028415X.2022.2131062}},
doi = {{10.1080/1028415X.2022.2131062}},
volume = {{26}},
year = {{2023}},
}