Impact of storage time prior to cryopreservation on mechanical properties of aortic homografts
Axelsson, Ida LU ; Gustafsson, Anna LU
; Isaksson, Hanna
LU
; Nilsson, Johan
LU
and Malm, Torsten
LU
(2023)
In Cell and Tissue Banking
- Abstract
Optimal time spans in homograft procurement are still debatable among tissue banks and needs to be further investigated. Cell viability decreases at longer preparation intervals, but the effect on collagen and elastic fibers has not been investigated to the same extent. These fibers are of importance to the homograft elasticity and strength. The objective of this study was to analyze the mechanical properties of homograft tissue at different time spans in the procurement process. Ten aortic homografts were collected at the Tissue Bank in Lund. Twelve samples were obtained from each homograft, cryopreserved in groups of three after 2–4 days, 7–9 days, 28–30 days, and 60–62 days in antibiotic decontamination. Mechanical testing was... (More)
Optimal time spans in homograft procurement are still debatable among tissue banks and needs to be further investigated. Cell viability decreases at longer preparation intervals, but the effect on collagen and elastic fibers has not been investigated to the same extent. These fibers are of importance to the homograft elasticity and strength. The objective of this study was to analyze the mechanical properties of homograft tissue at different time spans in the procurement process. Ten aortic homografts were collected at the Tissue Bank in Lund. Twelve samples were obtained from each homograft, cryopreserved in groups of three after 2–4 days, 7–9 days, 28–30 days, and 60–62 days in antibiotic decontamination. Mechanical testing was performed with uniaxial tensile tests, calculating elastic modulus, yield stress and energy at yield stress. Two randomly selected samples were assessed with light microscopy. Procurement generated a total of 120 samples, with 30 samples in each time group. Elastic modulus and yield stress was significantly higher in samples cryopreserved after 2–4 days (2.7 MPa (2.5-5.0) and 0.78 MPa (0.68-1.0)) compared to 7–9 days (2.2 MPa (2.0-2.6) and 0.53 MPa (0.46–0.69)), p = 0.008 and 0.011 respectively. Light microscopy did not show any difference in collagen and elastin at different time spans. There was a significant decrease in elastic modulus and yield stress after 7 days of decontamination at 4 °C compared to 2–4 days. This could indicate some deterioration of elastin and collagen at longer decontamination intervals. Clinical significance of these findings remains to be clarified.
(Less)
- author
- Axelsson, Ida
LU
; Gustafsson, Anna
LU
; Isaksson, Hanna
LU
; Nilsson, Johan
LU
and Malm, Torsten
LU
- organization
-
- Artificial Intelligence and Bioinformatics in Cardiothoracic Sciences (AIBCTS) (research group)
- Thoracic Surgery
- Department of Biomedical Engineering
- LTH Profile Area: Engineering Health
- Heart and Lung transplantation (research group)
- eSSENCE: The e-Science Collaboration
- Heparin bindning protein in cardiothoracic surgery (research group)
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- epub
- subject
- keywords
- Congenital cardiac surgery, Elastic modulus, Homografts, Yield stress
- in
- Cell and Tissue Banking
- publisher
- Springer
- external identifiers
-
- pmid:36843158
- scopus:85148875739
- ISSN
- 1389-9333
- DOI
- 10.1007/s10561-023-10079-z
- language
- English
- LU publication?
- yes
- id
- ff3dae7f-b3ac-4f71-9b45-8205258ba685
- date added to LUP
- 2023-03-16 15:26:15
- date last changed
- 2024-06-13 10:14:21
@article{ff3dae7f-b3ac-4f71-9b45-8205258ba685,
abstract = {{<p>Optimal time spans in homograft procurement are still debatable among tissue banks and needs to be further investigated. Cell viability decreases at longer preparation intervals, but the effect on collagen and elastic fibers has not been investigated to the same extent. These fibers are of importance to the homograft elasticity and strength. The objective of this study was to analyze the mechanical properties of homograft tissue at different time spans in the procurement process. Ten aortic homografts were collected at the Tissue Bank in Lund. Twelve samples were obtained from each homograft, cryopreserved in groups of three after 2–4 days, 7–9 days, 28–30 days, and 60–62 days in antibiotic decontamination. Mechanical testing was performed with uniaxial tensile tests, calculating elastic modulus, yield stress and energy at yield stress. Two randomly selected samples were assessed with light microscopy. Procurement generated a total of 120 samples, with 30 samples in each time group. Elastic modulus and yield stress was significantly higher in samples cryopreserved after 2–4 days (2.7 MPa (2.5-5.0) and 0.78 MPa (0.68-1.0)) compared to 7–9 days (2.2 MPa (2.0-2.6) and 0.53 MPa (0.46–0.69)), p = 0.008 and 0.011 respectively. Light microscopy did not show any difference in collagen and elastin at different time spans. There was a significant decrease in elastic modulus and yield stress after 7 days of decontamination at 4 °C compared to 2–4 days. This could indicate some deterioration of elastin and collagen at longer decontamination intervals. Clinical significance of these findings remains to be clarified.</p>}},
author = {{Axelsson, Ida and Gustafsson, Anna and Isaksson, Hanna and Nilsson, Johan and Malm, Torsten}},
issn = {{1389-9333}},
keywords = {{Congenital cardiac surgery; Elastic modulus; Homografts; Yield stress}},
language = {{eng}},
publisher = {{Springer}},
series = {{Cell and Tissue Banking}},
title = {{Impact of storage time prior to cryopreservation on mechanical properties of aortic homografts}},
url = {{http://dx.doi.org/10.1007/s10561-023-10079-z}},
doi = {{10.1007/s10561-023-10079-z}},
year = {{2023}},
}