Exogenous LL-37 but not homogenates of desquamated oral epithelial cells shows activity against Streptococcus mutans
(2021) In Acta Odontologica Scandinavica 79(6). p.466-472- Abstract
Objective: The antimicrobial peptide hCAP18/LL-37 is detected in desquamated epithelial cells of human whole saliva, but the functional importance of this pool of hCAP18/LL-37 is not understood. Here, we assess the impact of homogenates of desquamated oral epithelial cells and exogenous, synthetic LL-37 on two oral bacteria: S. mutans and S. gordonii. Material and methods: Desquamated epithelial cells of unstimulated whole saliva were isolated and cellular and extracellular levels of hCAP18/LL-37 analyzed by ELISA. Bacterial viability was determined by BacLight Live/Dead staining and confocal laser scanning microscopy. Results: Desquamated oral epithelial cells harboured hCAP18/LL-37, and they spontaneously released/leaked the peptide... (More)
Objective: The antimicrobial peptide hCAP18/LL-37 is detected in desquamated epithelial cells of human whole saliva, but the functional importance of this pool of hCAP18/LL-37 is not understood. Here, we assess the impact of homogenates of desquamated oral epithelial cells and exogenous, synthetic LL-37 on two oral bacteria: S. mutans and S. gordonii. Material and methods: Desquamated epithelial cells of unstimulated whole saliva were isolated and cellular and extracellular levels of hCAP18/LL-37 analyzed by ELISA. Bacterial viability was determined by BacLight Live/Dead staining and confocal laser scanning microscopy. Results: Desquamated oral epithelial cells harboured hCAP18/LL-37, and they spontaneously released/leaked the peptide to their medium. Exogenous, synthetic LL-37 showed cytotoxic activity against S. mutans but not S gordonii, suggesting that LL-37 acts differentially on these two types of oral bacteria. Homogenates of desquamated oral epithelial cells had no effect on S. mutans viability. Treatment with exogenous, synthetic LL-37 (8 and 10 μM) reduced S. mutans viability, whereas lower concentrations (0.1 and 1 µM) of the peptide lacked effect. Conclusions: Desquamated oral epithelial cells contain hCAP18/LL-37, but their cellular levels of hCAP18/LL-37 are too low to affect S. mutans viability, whereas exogenous, synthetic LL-37 has a strong effect on these bacteria.
(Less)
- author
- Aidoukovitch, Alexandra
LU
; Bankell, Elisabeth
LU
; Davies, Julia R.
and Nilsson, Bengt Olof
LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cathelicidin, host defense peptide, innate immunity, oral bacteria, saliva
- in
- Acta Odontologica Scandinavica
- volume
- 79
- issue
- 6
- pages
- 466 - 472
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:85102250279
- pmid:33687301
- ISSN
- 0001-6357
- DOI
- 10.1080/00016357.2021.1892180
- project
- Production and transport of the antimicrobial peptide LL-37 and other peptides in saliva: physiological and pathophysiological importance
- language
- English
- LU publication?
- yes
- id
- ff5f7bb2-3d2c-4087-bb90-e4d7d65f8699
- date added to LUP
- 2021-03-26 11:24:14
- date last changed
- 2025-01-26 07:47:48
@article{ff5f7bb2-3d2c-4087-bb90-e4d7d65f8699, abstract = {{<p>Objective: The antimicrobial peptide hCAP18/LL-37 is detected in desquamated epithelial cells of human whole saliva, but the functional importance of this pool of hCAP18/LL-37 is not understood. Here, we assess the impact of homogenates of desquamated oral epithelial cells and exogenous, synthetic LL-37 on two oral bacteria: S. mutans and S. gordonii. Material and methods: Desquamated epithelial cells of unstimulated whole saliva were isolated and cellular and extracellular levels of hCAP18/LL-37 analyzed by ELISA. Bacterial viability was determined by BacLight Live/Dead staining and confocal laser scanning microscopy. Results: Desquamated oral epithelial cells harboured hCAP18/LL-37, and they spontaneously released/leaked the peptide to their medium. Exogenous, synthetic LL-37 showed cytotoxic activity against S. mutans but not S gordonii, suggesting that LL-37 acts differentially on these two types of oral bacteria. Homogenates of desquamated oral epithelial cells had no effect on S. mutans viability. Treatment with exogenous, synthetic LL-37 (8 and 10 μM) reduced S. mutans viability, whereas lower concentrations (0.1 and 1 µM) of the peptide lacked effect. Conclusions: Desquamated oral epithelial cells contain hCAP18/LL-37, but their cellular levels of hCAP18/LL-37 are too low to affect S. mutans viability, whereas exogenous, synthetic LL-37 has a strong effect on these bacteria.</p>}}, author = {{Aidoukovitch, Alexandra and Bankell, Elisabeth and Davies, Julia R. and Nilsson, Bengt Olof}}, issn = {{0001-6357}}, keywords = {{Cathelicidin; host defense peptide; innate immunity; oral bacteria; saliva}}, language = {{eng}}, number = {{6}}, pages = {{466--472}}, publisher = {{Taylor & Francis}}, series = {{Acta Odontologica Scandinavica}}, title = {{Exogenous LL-37 but not homogenates of desquamated oral epithelial cells shows activity against Streptococcus mutans}}, url = {{http://dx.doi.org/10.1080/00016357.2021.1892180}}, doi = {{10.1080/00016357.2021.1892180}}, volume = {{79}}, year = {{2021}}, }