Immunohistochemical analysis of cannabinoid receptor 1 expression in steatotic rat livers
(2016) In Experimental and Therapeutic Medicine 11(4). p.1227-1230- Abstract
The primary aim of the present study was to determine the expression levels of cannabinoid receptor type 1 (CB1) in steatotic rat livers. The secondary aim was to clarify whether steatosis and inflammation are more marked in areas with increased CB1 overexpression. For ethical and economic reasons, the present study investigated tissue from archived liver blocks, which were obtained from 38 rats that had been euthanized during the course of previous research at the Karolinska Institute of the Karolinska University Hospital (Stockholm, Sweden) and Lund University (Malmö, Sweden). Liver tissue fixed in formalin and embedded in paraffin was used that had been sourced from 36 male Sprague Dawley rats (age, 7 weeks) and 2 rats (age, 180... (More)
The primary aim of the present study was to determine the expression levels of cannabinoid receptor type 1 (CB1) in steatotic rat livers. The secondary aim was to clarify whether steatosis and inflammation are more marked in areas with increased CB1 overexpression. For ethical and economic reasons, the present study investigated tissue from archived liver blocks, which were obtained from 38 rats that had been euthanized during the course of previous research at the Karolinska Institute of the Karolinska University Hospital (Stockholm, Sweden) and Lund University (Malmö, Sweden). Liver tissue fixed in formalin and embedded in paraffin was used that had been sourced from 36 male Sprague Dawley rats (age, 7 weeks) and 2 rats (age, 180 days) lacking normal leptin receptors. The rat liver tissue was stained with antibodies against CB1 and counterstained with hematoxylin. The expression of CB1 and the number of cells overexpressing CB1 were determined. Steatosis was scored according to the Dixon scoring system. CB1 overexpression and steatosis were detected in hepatocytes from all 38 livers sampled. The expression of CB1 was more marked in hepatocytes localized next to portal triads. Near the central veins, the expression was significantly weaker. Steatosis was more marked in areas of increased CB1 overexpres with publications reporting that CB1 in hepatocytes increases lipogenesis and contributes to inflammation.
(Less)
- author
- Zduniak, Krzysztof ; Ziółkowski, Piotr ; Regnell, Pontus ; Tollet-Egnell, Petra ; Åkesson, Lina LU and Cooper, Martin E.
- organization
- publishing date
- 2016-04-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cannabinoid receptor 1, Fatty liver, Immunohistochemistry, Rats
- in
- Experimental and Therapeutic Medicine
- volume
- 11
- issue
- 4
- pages
- 4 pages
- publisher
- Spandidos Publications
- external identifiers
-
- pmid:27073427
- wos:000372709800012
- scopus:84958778487
- ISSN
- 1792-0981
- DOI
- 10.3892/etm.2016.3036
- language
- English
- LU publication?
- yes
- id
- 0970f3b8-b1fa-4ab7-a331-a367e81ec720
- date added to LUP
- 2016-07-25 14:24:59
- date last changed
- 2024-10-04 23:29:39
@article{0970f3b8-b1fa-4ab7-a331-a367e81ec720, abstract = {{<p>The primary aim of the present study was to determine the expression levels of cannabinoid receptor type 1 (CB1) in steatotic rat livers. The secondary aim was to clarify whether steatosis and inflammation are more marked in areas with increased CB1 overexpression. For ethical and economic reasons, the present study investigated tissue from archived liver blocks, which were obtained from 38 rats that had been euthanized during the course of previous research at the Karolinska Institute of the Karolinska University Hospital (Stockholm, Sweden) and Lund University (Malmö, Sweden). Liver tissue fixed in formalin and embedded in paraffin was used that had been sourced from 36 male Sprague Dawley rats (age, 7 weeks) and 2 rats (age, 180 days) lacking normal leptin receptors. The rat liver tissue was stained with antibodies against CB1 and counterstained with hematoxylin. The expression of CB1 and the number of cells overexpressing CB1 were determined. Steatosis was scored according to the Dixon scoring system. CB1 overexpression and steatosis were detected in hepatocytes from all 38 livers sampled. The expression of CB1 was more marked in hepatocytes localized next to portal triads. Near the central veins, the expression was significantly weaker. Steatosis was more marked in areas of increased CB1 overexpres with publications reporting that CB1 in hepatocytes increases lipogenesis and contributes to inflammation.</p>}}, author = {{Zduniak, Krzysztof and Ziółkowski, Piotr and Regnell, Pontus and Tollet-Egnell, Petra and Åkesson, Lina and Cooper, Martin E.}}, issn = {{1792-0981}}, keywords = {{Cannabinoid receptor 1; Fatty liver; Immunohistochemistry; Rats}}, language = {{eng}}, month = {{04}}, number = {{4}}, pages = {{1227--1230}}, publisher = {{Spandidos Publications}}, series = {{Experimental and Therapeutic Medicine}}, title = {{Immunohistochemical analysis of cannabinoid receptor 1 expression in steatotic rat livers}}, url = {{http://dx.doi.org/10.3892/etm.2016.3036}}, doi = {{10.3892/etm.2016.3036}}, volume = {{11}}, year = {{2016}}, }