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Accelerated glucose discoloration method - A quick tool for glucose stability assessment

Scret, Cristina LU (2011) KEMR16 20102
Department of Chemistry
Abstract
The non-enzymatic browning of glucose was investigated by accelerating the glucose degradation with or without heating glucose solutions for 1 h at 100°C with different reagents. Evaluation of the glucose degradation was performed using two types of glucose, glucose A and glucose B, in order to investigate the influence of the glucose manufacturing process on glucose discoloration. The color formation was determined by measuring the UV-absorbance between 260-605 nm. The glucose degradation was also investigated by the formation of 5-hydroxymethylfurfural and 2-furfural, which were determined by HPLC.
The accelerated glucose discoloration method was optimised in order to differentiate between different glucose qualities with respect to... (More)
The non-enzymatic browning of glucose was investigated by accelerating the glucose degradation with or without heating glucose solutions for 1 h at 100°C with different reagents. Evaluation of the glucose degradation was performed using two types of glucose, glucose A and glucose B, in order to investigate the influence of the glucose manufacturing process on glucose discoloration. The color formation was determined by measuring the UV-absorbance between 260-605 nm. The glucose degradation was also investigated by the formation of 5-hydroxymethylfurfural and 2-furfural, which were determined by HPLC.
The accelerated glucose discoloration method was optimised in order to differentiate between different glucose qualities with respect to color stability, i.e. the conditions selected were based on maximizing the difference in glucose discoloration for glucose A and B. Direct measurement of the absorbance difference at 350 nm, on 33 % (w/w) glucose/water solution kept at room temperature for 1 h was found to be the optimum conditions for differentiating between glucose A an B. Further investigation on Maillard reactions was made, and for the investigated conditions, the reaction was found to be most accelerated for the glucose samples, 33% (w/w), heated at 100°C for 1 hour in the presence of L-alanine. (Less)
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author
Scret, Cristina LU
supervisor
organization
course
KEMR16 20102
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Analytisk kemi
language
English
id
2863036
date added to LUP
2012-08-14 14:31:45
date last changed
2012-08-14 14:31:45
@misc{2863036,
  abstract     = {The non-enzymatic browning of glucose was investigated by accelerating the glucose degradation with or without heating glucose solutions for 1 h at 100°C with different reagents. Evaluation of the glucose degradation was performed using two types of glucose, glucose A and glucose B, in order to investigate the influence of the glucose manufacturing process on glucose discoloration. The color formation was determined by measuring the UV-absorbance between 260-605 nm. The glucose degradation was also investigated by the formation of 5-hydroxymethylfurfural and 2-furfural, which were determined by HPLC.
The accelerated glucose discoloration method was optimised in order to differentiate between different glucose qualities with respect to color stability, i.e. the conditions selected were based on maximizing the difference in glucose discoloration for glucose A and B. Direct measurement of the absorbance difference at 350 nm, on 33 % (w/w) glucose/water solution kept at room temperature for 1 h was found to be the optimum conditions for differentiating between glucose A an B. Further investigation on Maillard reactions was made, and for the investigated conditions, the reaction was found to be most accelerated for the glucose samples, 33% (w/w), heated at 100°C for 1 hour in the presence of L-alanine.},
  author       = {Scret, Cristina},
  keyword      = {Analytisk kemi},
  language     = {eng},
  note         = {Student Paper},
  title        = {Accelerated glucose discoloration method - A quick tool for glucose stability assessment},
  year         = {2011},
}