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Metabolic engineering of Yarrowia lipolytica for production of triacetic acid lactone

Landberg, Jenny LU (2016) KMB820 20152
Applied Microbiology
Biotechnology
Abstract
Climate change and global warming is driving development toward use of more
sustainable production processes such as microbial manufacturing of renewable
compounds. Yarrowia lipolytica is a non-conventional, oleaginous yeast that can
accumulate high amounts of lipids in response to stress conditions, and is therefore
of special interest in the biotechnological industry. The goal with this study was to
harness the inherent oleaginous nature of Y. lipolytica for production of triacetic
acid lactone (TAL), a potential biorenewable platform chemical that is similar to fatty
acids in structure and synthesis. Several metabolic engineering approaches were
taken to improve TAL titer and understand mechanisms driving TAL... (More)
Climate change and global warming is driving development toward use of more
sustainable production processes such as microbial manufacturing of renewable
compounds. Yarrowia lipolytica is a non-conventional, oleaginous yeast that can
accumulate high amounts of lipids in response to stress conditions, and is therefore
of special interest in the biotechnological industry. The goal with this study was to
harness the inherent oleaginous nature of Y. lipolytica for production of triacetic
acid lactone (TAL), a potential biorenewable platform chemical that is similar to fatty
acids in structure and synthesis. Several metabolic engineering approaches were
taken to improve TAL titer and understand mechanisms driving TAL accumulation,
including introduction of a mutant enzyme in the glycolysis pathway, disruption of an
enzyme involved in precursor-production for lipids, and disruption of peroxisome
biogenesis. Furthermore, optimal nitrogen and carbon concentrations for
accumulation of TAL in Y. lipolytica were examined. This study also presents a
new method for elucidating gene essentiality using CRISPR-Cas9. (Less)
Popular Abstract (Swedish)
I projektet undesöktes hur man kan använda jästsvampen Yarrowia lipolytica för att tillverka en kemikalie som kallas TAL. Från TAL kan man tillverka andra ämnen som bland annat kan användas som konserveringsmedel i mat. Y. lipolytica är väldigt lik den bakjäst som vi använder för att göra bröd och bullar. En av skillnaderna från bakjäst är att Y. lipolytica lagrar sin överskottsenergi i form av fetter– precis som vi människor. Det är just den effektiva fett-tillverkning i Y. lipolytica som man vill utnyttja för att göra TAL. Tillverkningsstegen för TAL och fetter är nämligen väldigt lika, och katalyseras av liknande enzym. TAL tillverkas naturligt i blomman gerbera, av ett enzym som heter 2-pyronsyntas. Genom att klona in 2-pyronsyntas i... (More)
I projektet undesöktes hur man kan använda jästsvampen Yarrowia lipolytica för att tillverka en kemikalie som kallas TAL. Från TAL kan man tillverka andra ämnen som bland annat kan användas som konserveringsmedel i mat. Y. lipolytica är väldigt lik den bakjäst som vi använder för att göra bröd och bullar. En av skillnaderna från bakjäst är att Y. lipolytica lagrar sin överskottsenergi i form av fetter– precis som vi människor. Det är just den effektiva fett-tillverkning i Y. lipolytica som man vill utnyttja för att göra TAL. Tillverkningsstegen för TAL och fetter är nämligen väldigt lika, och katalyseras av liknande enzym. TAL tillverkas naturligt i blomman gerbera, av ett enzym som heter 2-pyronsyntas. Genom att klona in 2-pyronsyntas i Y. lipolytica kan man få den att tillverka TAL. I projektet användes olika metoder för att förstå vad som styr produktion av TAL, och om man kan öka produktionen genom att slå ut funktionen hos vissa gener. (Less)
Please use this url to cite or link to this publication:
author
Landberg, Jenny LU
supervisor
organization
course
KMB820 20152
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Yarrowia lipolytica, metabolic engineering, triacetic acid lactone
language
English
id
8878878
date added to LUP
2016-06-21 16:13:14
date last changed
2016-07-11 06:51:28
@misc{8878878,
  abstract     = {Climate change and global warming is driving development toward use of more
sustainable production processes such as microbial manufacturing of renewable
compounds. [i]Yarrowia lipolytica[/i] is a non-conventional, oleaginous yeast that can
accumulate high amounts of lipids in response to stress conditions, and is therefore
of special interest in the biotechnological industry. The goal with this study was to
harness the inherent oleaginous nature of [i]Y. lipolytica[/i] for production of triacetic
acid lactone (TAL), a potential biorenewable platform chemical that is similar to fatty
acids in structure and synthesis. Several metabolic engineering approaches were
taken to improve TAL titer and understand mechanisms driving TAL accumulation,
including introduction of a mutant enzyme in the glycolysis pathway, disruption of an
enzyme involved in precursor-production for lipids, and disruption of peroxisome
biogenesis. Furthermore, optimal nitrogen and carbon concentrations for
accumulation of TAL in [i]Y. lipolytica[/i] were examined. This study also presents a
new method for elucidating gene essentiality using CRISPR-Cas9.},
  author       = {Landberg, Jenny},
  keyword      = {Yarrowia lipolytica,metabolic engineering,triacetic acid lactone},
  language     = {eng},
  note         = {Student Paper},
  title        = {Metabolic engineering of Yarrowia lipolytica for production of triacetic acid lactone},
  year         = {2016},
}