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Studies on the mineral usage by bacterial pathogens

Ortenlöf, Niklas LU (2016) KEMP42 20161
Department of Chemistry
Abstract
Cofactors are essential for the growth of bacterial pathogens also inside a human host and one source of cofactors are proteins in blood. Pathogens can use surface proteins to bind and extract cofactors from these blood proteins. Here, using protein modeling of these proteins in complex with bacterial protein combined with bacterial growth assays with blood proteins from different species, we demonstrate that a certain bacterium preferably utilizes the one of the peptide chains of a specific blood protein for its growth. We also mapped a putative binding region in this blood protein. Moreover, redox status of these proteins appears to affect the growth of this bacterium in a dose-response manner. Unexpectedly, the key bacterial surface... (More)
Cofactors are essential for the growth of bacterial pathogens also inside a human host and one source of cofactors are proteins in blood. Pathogens can use surface proteins to bind and extract cofactors from these blood proteins. Here, using protein modeling of these proteins in complex with bacterial protein combined with bacterial growth assays with blood proteins from different species, we demonstrate that a certain bacterium preferably utilizes the one of the peptide chains of a specific blood protein for its growth. We also mapped a putative binding region in this blood protein. Moreover, redox status of these proteins appears to affect the growth of this bacterium in a dose-response manner. Unexpectedly, the key bacterial surface receptor is not essential for bacterial growth in presence of high concentrations of the blood protein. In conclusion, these results will be important in the design of novel therapies against bacterial pathogens. (Less)
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author
Ortenlöf, Niklas LU
supervisor
organization
course
KEMP42 20161
year
type
L3 - Miscellaneous, Projetcs etc.
subject
keywords
protein science, proteinvetenskap
language
English
id
8892457
date added to LUP
2017-07-13 11:24:43
date last changed
2017-07-13 11:24:43
@misc{8892457,
  abstract     = {Cofactors are essential for the growth of bacterial pathogens also inside a human host and one source of cofactors are proteins in blood. Pathogens can use surface proteins to bind and extract cofactors from these blood proteins. Here, using protein modeling of these proteins in complex with bacterial protein combined with bacterial growth assays with blood proteins from different species, we demonstrate that a certain bacterium preferably utilizes the one of the peptide chains of a specific blood protein for its growth. We also mapped a putative binding region in this blood protein. Moreover, redox status of these proteins appears to affect the growth of this bacterium in a dose-response manner. Unexpectedly, the key bacterial surface receptor is not essential for bacterial growth in presence of high concentrations of the blood protein. In conclusion, these results will be important in the design of novel therapies against bacterial pathogens.},
  author       = {Ortenlöf, Niklas},
  keyword      = {protein science,proteinvetenskap},
  language     = {eng},
  note         = {Student Paper},
  title        = {Studies on the mineral usage by bacterial pathogens},
  year         = {2016},
}