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Preparation and evaluation of arabinoxylan based prebiotics

Sajib, Mohammad Abu Mursalin LU (2019) In Preparation and evaluation of arabinoxylan based prebiotics KBTM01 20161
Biotechnology
Abstract
Prebiotics are non-digestible carbohydrates that selectively stimulate the growth and activity of health promoting bacteria in the colon, resulting in homeostasis to the gut environment. It is considered to be the solution of many health-related diseases such as type-2 diabetes, cardiovascular disease and cancer. Arabinoxylooligosaccharides, the hydrolysis product of arabinoxylan from plant cell walls, can be used as potential prebiotics as it is non-digestible to humans and can be fermented by the bacteria in the colon. Brewer’s spent grain, the residue from beer production, was used to extract arabinoxylan. The composition of brewer’s spent grain was analyzed and arabinoxylan content found to be 14.75% on a dry weight basis. Different... (More)
Prebiotics are non-digestible carbohydrates that selectively stimulate the growth and activity of health promoting bacteria in the colon, resulting in homeostasis to the gut environment. It is considered to be the solution of many health-related diseases such as type-2 diabetes, cardiovascular disease and cancer. Arabinoxylooligosaccharides, the hydrolysis product of arabinoxylan from plant cell walls, can be used as potential prebiotics as it is non-digestible to humans and can be fermented by the bacteria in the colon. Brewer’s spent grain, the residue from beer production, was used to extract arabinoxylan. The composition of brewer’s spent grain was analyzed and arabinoxylan content found to be 14.75% on a dry weight basis. Different pre-treatment methods were used to extract arabinoxylan-rich fractions from brewer’s spent grain. The best pre-treatment method was selected based on the purity of extracted arabinoxylan, simplicity of the process and possibility to use in large scale applications. The pre-treatment process simply consists of a heat-treatment step of the de-starched brewer’s spent grain and is followed by mild alkaline treatment. Xylanases were used to produce short-chain oligosaccharides from extracted arabinoxylan. Two pure xylanases namely RmXyn10A from GH family 10 and Pentopan Mono BG from GH family 11 were used to depict their product formation (hydrolysis) pattern. Meanwhile, seven other commercial xylanases were used to compare their efficiency in making short-chain oligosaccharides. Most of the commercial xylanases were found to be efficient in producing mono-saccharides (arabinose and xylose) while two pure xylanases were efficient in making short oligosaccharides. Well-established probiotic strain Lactobacillus brevis (DSMZ 1264) was used to perform growth studies by using arabinoxylooligosaccharides as carbon source. Analyzed results of samples after 48 hours of fermentation showed that oligosaccharides were utilized by Lactobacillus brevis. (Less)
Popular Abstract
Prebiotics, the non-digestible carbohydrate compounds, play an important role to selectively stimulate the growth and activity of health promoting gut microorganisms. Previous research findings have proved that the consumption of prebiotics improves the reduction of blood glucose and cholesterol, enhances mineral absorption from the colon, deactivates pro-carcinogenic enzymes in the gastrointestinal tract as well as their stimulation of the immune system.


By definition, any non-digestible carbohydrates can be considered as prebiotics. Some of the commercially available prebiotics nowadays are inulin, fructooligosaccharide, galactooligosaccharide and guar-gum. Also, products containing xylooligosaccharides (XOS) and... (More)
Prebiotics, the non-digestible carbohydrate compounds, play an important role to selectively stimulate the growth and activity of health promoting gut microorganisms. Previous research findings have proved that the consumption of prebiotics improves the reduction of blood glucose and cholesterol, enhances mineral absorption from the colon, deactivates pro-carcinogenic enzymes in the gastrointestinal tract as well as their stimulation of the immune system.


By definition, any non-digestible carbohydrates can be considered as prebiotics. Some of the commercially available prebiotics nowadays are inulin, fructooligosaccharide, galactooligosaccharide and guar-gum. Also, products containing xylooligosaccharides (XOS) and arabinoxylooligosaccharides (AXOS) are considered as the potential future prebiotics in the market. This XOS and AXOS are the hydrolysis products of xylan and xylan is the most abundantly available cell wall component of lignocellulosic biomass. Agricultural wastes such as straw, husk, stalk, cob, hull, bagasse and pulp of hard wood have become a major source of xylan. Productions of XOS and AXOS from agricultural residues offer an excellent prospect to the nutraceutical industries owing to the low price and abundant availability of the raw materials.


Brewer’s spent grain (BSG) is the production residue from the process of malts (cereal grains) and the separation of wort (fermentation medium to produce beer) during the beer production. BSG is the most abundant by-product generated by brewing industry and accounts for ca. 85% of total by-products. Arabinoxylan, lignin and cellulose are the most abundant compounds in BSG where they weigh roughly 28%, 28% and 17%, respectively. The most common applications of BSG are being used as animal feed and dumping into landfills. This research study was carried out as an attempt to produce AXOS from BSG which is believed to be a source of great potential for making prebiotics. A new technique was developed and presented in this study to extract arabinoxylan from BSG. This technique is compatible to be used in the large scale production of arabinoxylan. Different xylanases were used to hydrolyze the extracted arabinoxylan fractions and to produce short-chain AXOS. Also, in-vitro fermentation experiment was carried out to assess the capability of extracted BSG AXOS to be used by health promoting gut bacteria Lactobacillus. In-vitro fermentation of extracted BSG AXOS fractions using well-known probiotic strain Lactobacillus showed the production of short-chain fatty acids.


This study showed that AXOS from BSG have the potential to be utilized by probiotic bacteria and to produce short-chain fatty acids. The technique developed in this study to extract arabinoxylan from BSG can be applied for large scale production of arabinoxylan. Furthermore, the production residues after arabinoxylan extraction can be used as animal feed. (Less)
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author
Sajib, Mohammad Abu Mursalin LU
supervisor
organization
course
KBTM01 20161
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Arabinoxylan, Probiotics, Prebiotics, Food, Xylanase, Brewer's spent grain, Biotechnology, Bioteknik
publication/series
Preparation and evaluation of arabinoxylan based prebiotics
language
English
id
8903875
date added to LUP
2017-03-24 09:09:36
date last changed
2017-03-24 09:51:37
@misc{8903875,
  abstract     = {Prebiotics are non-digestible carbohydrates that selectively stimulate the growth and activity of health promoting bacteria in the colon, resulting in homeostasis to the gut environment. It is considered to be the solution of many health-related diseases such as type-2 diabetes, cardiovascular disease and cancer. Arabinoxylooligosaccharides, the hydrolysis product of arabinoxylan from plant cell walls, can be used as potential prebiotics as it is non-digestible to humans and can be fermented by the bacteria in the colon. Brewer’s spent grain, the residue from beer production, was used to extract arabinoxylan. The composition of brewer’s spent grain was analyzed and arabinoxylan content found to be 14.75% on a dry weight basis. Different pre-treatment methods were used to extract arabinoxylan-rich fractions from brewer’s spent grain. The best pre-treatment method was selected based on the purity of extracted arabinoxylan, simplicity of the process and possibility to use in large scale applications. The pre-treatment process simply consists of a heat-treatment step of the de-starched brewer’s spent grain and is followed by mild alkaline treatment. Xylanases were used to produce short-chain oligosaccharides from extracted arabinoxylan. Two pure xylanases namely RmXyn10A from GH family 10 and Pentopan Mono BG from GH family 11 were used to depict their product formation (hydrolysis) pattern. Meanwhile, seven other commercial xylanases were used to compare their efficiency in making short-chain oligosaccharides. Most of the commercial xylanases were found to be efficient in producing mono-saccharides (arabinose and xylose) while two pure xylanases were efficient in making short oligosaccharides. Well-established probiotic strain Lactobacillus brevis (DSMZ 1264) was used to perform growth studies by using arabinoxylooligosaccharides as carbon source. Analyzed results of samples after 48 hours of fermentation showed that oligosaccharides were utilized by Lactobacillus brevis.},
  author       = {Sajib, Mohammad Abu Mursalin},
  keyword      = {Arabinoxylan,Probiotics,Prebiotics,Food,Xylanase,Brewer's spent grain,Biotechnology,Bioteknik},
  language     = {eng},
  note         = {Student Paper},
  series       = {Preparation and evaluation of arabinoxylan based prebiotics},
  title        = {Preparation and evaluation of arabinoxylan based prebiotics},
  year         = {2019},
}