Impact of glycosaminoglycans on lung cells cultured on a macroporous hydrogel scaffold
(2018) KBKM05 20181Pure and Applied Biochemistry
Computational Chemistry
- Abstract
- The demand of healthy functional lungs is increasing, requiring a greater need of knowledge about lung tissue regeneration and repair. Glycosaminoglycans (GAGs) – polysaccharide sidechains found on proteins in the extracellular matrix (ECM) – are believed to be involved in several processes in the lung including cell proliferation, cell differentiation and tissue regeneration. We have developed a macroporous hydrogel (cryogel) scaffold as a model system that can easily be modified with different types of GAGs to study their impact on cellular behavior. The synthetic scaffold offers a 3D environment mimicking the ECM with minimal interference in our analyses. Our aim is to find GAGs that promote lung tissue regeneration.
The cryogels (Ø... (More) - The demand of healthy functional lungs is increasing, requiring a greater need of knowledge about lung tissue regeneration and repair. Glycosaminoglycans (GAGs) – polysaccharide sidechains found on proteins in the extracellular matrix (ECM) – are believed to be involved in several processes in the lung including cell proliferation, cell differentiation and tissue regeneration. We have developed a macroporous hydrogel (cryogel) scaffold as a model system that can easily be modified with different types of GAGs to study their impact on cellular behavior. The synthetic scaffold offers a 3D environment mimicking the ECM with minimal interference in our analyses. Our aim is to find GAGs that promote lung tissue regeneration.
The cryogels (Ø 0.5 cm x 1 cm) were produced using free radical polymerization at partly frozen condition with two main materials – Hydroxyethyl methacrylate and Acrylamide. The cryogels were sliced into 1 mm discs before modified with the desired GAG – Chondroitin sulfate (CS) or Chondroitin Dermatan sulfate (CS/DS). GAG attachment was confirmed by disaccharide analysis. The modified scaffolds were seeded with lung fibroblasts and cultured for 24 hours before gel structure and cell behavior were analyzed. Prior to modification, the non-modified scaffolds were evaluated for cell culture during 7 days.
From our results we may conclude that the cryogel is a suitable model system for studying the impact of GAGs on lung fibroblasts. The cells were able to attach and spread on the material and the GAGs do affect cell behavior differently depending on the specific GAG and concentration used. Future work may include optimization of the cryogel as well as evaluating different combinations of GAGs and pulmonary cells to find cues promoting lung tissue regeneration. (Less) - Popular Abstract (Swedish)
- Vad gör vi när avgaser och cigarettrök förstört våra lungor? Vad gör vi när behovet av friska lungdonatorer blir för stort? Vi försöker tillverka lungor i labbet såklart!
Våra lungor utsätts dagligen för avgaser och andra skadliga ämnen som finns i luften runt omkring oss. För det mesta kan kroppen reparera skadan på egen hand, men allteftersom påfrestningarna blir för stora höjs risken att misslyckas. Sjukdomar såsom kroniskt obstruktiv lungsjukdom (KOL) och idiopatisk lungfibros har ökat de senaste åren och den enda behandlingen i slutändan är att göra en lungtransplantation. Det finns dessvärre inte tillräckligt med friska donatorer och dessutom innebär transplantationer ofta andra problem såsom bortstötning av organet. Ett... (More) - Vad gör vi när avgaser och cigarettrök förstört våra lungor? Vad gör vi när behovet av friska lungdonatorer blir för stort? Vi försöker tillverka lungor i labbet såklart!
Våra lungor utsätts dagligen för avgaser och andra skadliga ämnen som finns i luften runt omkring oss. För det mesta kan kroppen reparera skadan på egen hand, men allteftersom påfrestningarna blir för stora höjs risken att misslyckas. Sjukdomar såsom kroniskt obstruktiv lungsjukdom (KOL) och idiopatisk lungfibros har ökat de senaste åren och den enda behandlingen i slutändan är att göra en lungtransplantation. Det finns dessvärre inte tillräckligt med friska donatorer och dessutom innebär transplantationer ofta andra problem såsom bortstötning av organet. Ett alternativ till transplantation tror forskare kan vara att ”odla” ny lungvävnad utanför kroppen – i labbet! (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/8952976
- author
- Petersson, Alexandra LU
- supervisor
- organization
- alternative title
- Glykosaminoglykaners påverkan på lungceller odlade på en makroporös scaffold
- course
- KBKM05 20181
- year
- 2018
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- tissue engineering, medicine, lung biology, chemistry, polymer, glycosaminoglycans, biotechnology, engineering, biochemistry, applied biochemistry, tillämpad biokemi
- language
- English
- id
- 8952976
- date added to LUP
- 2018-06-27 12:45:03
- date last changed
- 2018-06-27 12:45:03
@misc{8952976,
abstract = {{The demand of healthy functional lungs is increasing, requiring a greater need of knowledge about lung tissue regeneration and repair. Glycosaminoglycans (GAGs) – polysaccharide sidechains found on proteins in the extracellular matrix (ECM) – are believed to be involved in several processes in the lung including cell proliferation, cell differentiation and tissue regeneration. We have developed a macroporous hydrogel (cryogel) scaffold as a model system that can easily be modified with different types of GAGs to study their impact on cellular behavior. The synthetic scaffold offers a 3D environment mimicking the ECM with minimal interference in our analyses. Our aim is to find GAGs that promote lung tissue regeneration.
The cryogels (Ø 0.5 cm x 1 cm) were produced using free radical polymerization at partly frozen condition with two main materials – Hydroxyethyl methacrylate and Acrylamide. The cryogels were sliced into 1 mm discs before modified with the desired GAG – Chondroitin sulfate (CS) or Chondroitin Dermatan sulfate (CS/DS). GAG attachment was confirmed by disaccharide analysis. The modified scaffolds were seeded with lung fibroblasts and cultured for 24 hours before gel structure and cell behavior were analyzed. Prior to modification, the non-modified scaffolds were evaluated for cell culture during 7 days.
From our results we may conclude that the cryogel is a suitable model system for studying the impact of GAGs on lung fibroblasts. The cells were able to attach and spread on the material and the GAGs do affect cell behavior differently depending on the specific GAG and concentration used. Future work may include optimization of the cryogel as well as evaluating different combinations of GAGs and pulmonary cells to find cues promoting lung tissue regeneration.}},
author = {{Petersson, Alexandra}},
language = {{eng}},
note = {{Student Paper}},
title = {{Impact of glycosaminoglycans on lung cells cultured on a macroporous hydrogel scaffold}},
year = {{2018}},
}