Changes in bacterial composition after consumption of a probiotic product against common colds
(2022) KLGM01 20221Food Technology and Nutrition (M.Sc.)
- Abstract
- The common cold may affect an individual's life, or socio-economic development. This study investigated the effect of a probiotic product (mixed strains) on colds and changes in the oral microbiome. The study included 45 subjects, 23 taking the probiotic product (probiotic groups) and 22 taking the placebo (placebo-control group). The number of colds and duration of colds were investigated separately for the two groups of subjects and saliva samples were collected for 16S rRNA gene sequencing to study the bacterial community at the beginning and end of the experiment for both groups. By analysing questionnaires from participants and analysing the sequence from NGS using QIIME2 revealed that there was no significant difference in the number... (More)
- The common cold may affect an individual's life, or socio-economic development. This study investigated the effect of a probiotic product (mixed strains) on colds and changes in the oral microbiome. The study included 45 subjects, 23 taking the probiotic product (probiotic groups) and 22 taking the placebo (placebo-control group). The number of colds and duration of colds were investigated separately for the two groups of subjects and saliva samples were collected for 16S rRNA gene sequencing to study the bacterial community at the beginning and end of the experiment for both groups. By analysing questionnaires from participants and analysing the sequence from NGS using QIIME2 revealed that there was no significant difference in the number of colds and duration of colds between the probiotic and control group. There were no significant changes in Alpha diversity and Beta diversity measures, and there was also no substantial significant difference in the relative abundance of the flora between the two groups. This suggests that the probiotic product may not change the composition and structure of oral microbiota. There was also a very small increase trend in the number of Bifidobacterium in saliva samples after the consumption of the probiotic product. In contrast, a slight decrease trend was observed in the number of Lactobacillus spp. (Less)
- Popular Abstract
- This study revealed that the probiotic product used in this experiment had no significant effect on common colds.
Main Text
The trial was designed as a randomized controlled clinical trial by analyzing 80 DNA samples from the saliva of the test subjects using NGS technology. It was found that the probiotic products used in the trial did not have a significant effect on the common cold. And there was no significant difference observed in Alpha and Beta diversity between the probiotic and control groups. It was indicated that the probiotic product used in this experiment might not have an effect on the composition and structure of oral microbiome. An interested result was found that there was no substantial difference observed in the... (More) - This study revealed that the probiotic product used in this experiment had no significant effect on common colds.
Main Text
The trial was designed as a randomized controlled clinical trial by analyzing 80 DNA samples from the saliva of the test subjects using NGS technology. It was found that the probiotic products used in the trial did not have a significant effect on the common cold. And there was no significant difference observed in Alpha and Beta diversity between the probiotic and control groups. It was indicated that the probiotic product used in this experiment might not have an effect on the composition and structure of oral microbiome. An interested result was found that there was no substantial difference observed in the relative abundance of bacteria microbiota between the probiotic and control groups.
The experiment was designed to address several questions and needs by addressing the following. l Whether probiotic products reduced the duration and number of episodes of the common cold l Whether the intake of probiotic products had a significant effect on the oral microbiome
l Give more evidence and conclusions about the effect of probiotics on colds
Through the analysis of NGS technology, these questions and needs were addressed and provide more evidence and information for future studies of probiotics on colds, particularly the effect of probiotic products on the bacterial composition of saliva in relation to common colds.
There were also some limitations in this study.
l Short duration of the probiotic product intake
l The probiotic product chosen was mixed strains and not specific strain.
l This study did not investigate which specific probiotic strains had an effect on the common
cold. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9088891
- author
- Wang, Tao LU
- supervisor
- organization
- alternative title
- Changes in the bacterial composition of saliva samples after consumption of a probiotic product against common colds
- course
- KLGM01 20221
- year
- 2022
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- probiotic, common cold, oral microbiome, food technology
- language
- English
- id
- 9088891
- date added to LUP
- 2022-06-17 08:43:26
- date last changed
- 2022-06-17 08:43:26
@misc{9088891,
abstract = {{The common cold may affect an individual's life, or socio-economic development. This study investigated the effect of a probiotic product (mixed strains) on colds and changes in the oral microbiome. The study included 45 subjects, 23 taking the probiotic product (probiotic groups) and 22 taking the placebo (placebo-control group). The number of colds and duration of colds were investigated separately for the two groups of subjects and saliva samples were collected for 16S rRNA gene sequencing to study the bacterial community at the beginning and end of the experiment for both groups. By analysing questionnaires from participants and analysing the sequence from NGS using QIIME2 revealed that there was no significant difference in the number of colds and duration of colds between the probiotic and control group. There were no significant changes in Alpha diversity and Beta diversity measures, and there was also no substantial significant difference in the relative abundance of the flora between the two groups. This suggests that the probiotic product may not change the composition and structure of oral microbiota. There was also a very small increase trend in the number of Bifidobacterium in saliva samples after the consumption of the probiotic product. In contrast, a slight decrease trend was observed in the number of Lactobacillus spp.}},
author = {{Wang, Tao}},
language = {{eng}},
note = {{Student Paper}},
title = {{Changes in bacterial composition after consumption of a probiotic product against common colds}},
year = {{2022}},
}