Starch-Based Aqueous Two-Phase Systems
(2023) KLGM15 20222Food Technology and Nutrition (M.Sc.)
- Abstract
- Using Aqueous Two-Phase Systems (ATPS) for e.g. the production of starch microspheres for protein or probiotic encapsulation is a gentle and cost-effective method with the potential to be used for sustained release systems. The main component of an ATPS is water and is in this thesis implemented based on the idea that two water-soluble polymers in solution might separate due to e.g. thermodynamic incompatibility, creating different phases in the system. The phases have a majority of water and one of the polymers in each phase, likely with a small trace of the other polymer.
In this thesis, the top phase contains the complementary polymer, and the bottom phase is the starch-rich phase. It is of interest to use starch as one of the... (More) - Using Aqueous Two-Phase Systems (ATPS) for e.g. the production of starch microspheres for protein or probiotic encapsulation is a gentle and cost-effective method with the potential to be used for sustained release systems. The main component of an ATPS is water and is in this thesis implemented based on the idea that two water-soluble polymers in solution might separate due to e.g. thermodynamic incompatibility, creating different phases in the system. The phases have a majority of water and one of the polymers in each phase, likely with a small trace of the other polymer.
In this thesis, the top phase contains the complementary polymer, and the bottom phase is the starch-rich phase. It is of interest to use starch as one of the polymers thanks to its ability to form particles when dispersed in ATPS. Different molecular weights of Poly(ethylene glycol) and Poly(ethylene oxide), were studied to see if other molecular weights could be used to form round, semi-crystalline microspheres. Changes in phase behavior for the different molecular weights and other complementary polymers, such as Hydroxypropyl methylcellulose, in the ATPS were also studied. By observing these different systems, it was possible to observe if phase separation occurred and, by hardening of the bottom phase, possible initial crystallization. To further investigate and map out the actual phase behavior a gravimetric water content quantification was carried out. Many complimentary polymers tested, favored a system that reached a desired water content of the bottom phase. It was shown that the production of starch microspheres in these systems are strongly dependent on the phase behavior of the polymers and the total weight concentration of polymers in the system. Furthermore, it was shown that the batch of starch and the method of mixing during incubation greatly influence the quality of the spheres. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9109267
- author
- Andersson, Josefine LU
- supervisor
- organization
- course
- KLGM15 20222
- year
- 2023
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Pharmaceutical technology, Starch Microspheres, Water-Soluble Polymers, Aqueous Two-Phase Systems
- language
- English
- id
- 9109267
- date added to LUP
- 2023-01-30 10:26:57
- date last changed
- 2023-01-30 10:26:57
@misc{9109267,
abstract = {{Using Aqueous Two-Phase Systems (ATPS) for e.g. the production of starch microspheres for protein or probiotic encapsulation is a gentle and cost-effective method with the potential to be used for sustained release systems. The main component of an ATPS is water and is in this thesis implemented based on the idea that two water-soluble polymers in solution might separate due to e.g. thermodynamic incompatibility, creating different phases in the system. The phases have a majority of water and one of the polymers in each phase, likely with a small trace of the other polymer.
In this thesis, the top phase contains the complementary polymer, and the bottom phase is the starch-rich phase. It is of interest to use starch as one of the polymers thanks to its ability to form particles when dispersed in ATPS. Different molecular weights of Poly(ethylene glycol) and Poly(ethylene oxide), were studied to see if other molecular weights could be used to form round, semi-crystalline microspheres. Changes in phase behavior for the different molecular weights and other complementary polymers, such as Hydroxypropyl methylcellulose, in the ATPS were also studied. By observing these different systems, it was possible to observe if phase separation occurred and, by hardening of the bottom phase, possible initial crystallization. To further investigate and map out the actual phase behavior a gravimetric water content quantification was carried out. Many complimentary polymers tested, favored a system that reached a desired water content of the bottom phase. It was shown that the production of starch microspheres in these systems are strongly dependent on the phase behavior of the polymers and the total weight concentration of polymers in the system. Furthermore, it was shown that the batch of starch and the method of mixing during incubation greatly influence the quality of the spheres.}},
author = {{Andersson, Josefine}},
language = {{eng}},
note = {{Student Paper}},
title = {{Starch-Based Aqueous Two-Phase Systems}},
year = {{2023}},
}