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Assessment Of Plastic Degrading Abilities Of Autotransporter Domains Containing Esterase On Synthetic Polyesters

Hansen, Linus LU (2023) KBTM05 20231
Biotechnology
Biotechnology (MSc)
Abstract
In today's world, plastic pollution has become a widespread problem with plastics in oceans,
rivers and forests. The use of plastic has increased rapidly since it first was developed and that is
because of its advantageous abilities. This scientific report focuses on the evaluation of two
enzymes known as "autotransporter domains containing esterase" for its ability to degrade a
variety of plastics such as polyethylene terephthalate (PET). In this report the ability to degrade
plastics with enzymes has been tested by introducing two different autotransporter domains
containing esterase to different kinds of plastic and letting them react under different conditions
to determine the plastic degrading abilities in the enzymes. The... (More)
In today's world, plastic pollution has become a widespread problem with plastics in oceans,
rivers and forests. The use of plastic has increased rapidly since it first was developed and that is
because of its advantageous abilities. This scientific report focuses on the evaluation of two
enzymes known as "autotransporter domains containing esterase" for its ability to degrade a
variety of plastics such as polyethylene terephthalate (PET). In this report the ability to degrade
plastics with enzymes has been tested by introducing two different autotransporter domains
containing esterase to different kinds of plastic and letting them react under different conditions
to determine the plastic degrading abilities in the enzymes. The results indicated that both
enzymes had an ability to degrade different plastics to some extent, but the extent of degradation
varied depending on the experimental conditions. However, the results were not entirely
conclusive as some of the results were contradictory. The biggest problem was replication of the
results, this can be due to several issues such as no expression of enzyme, problem with the
column and storage time of the enzyme. Despite the inconclusive results, the study provides
valuable insights into the potential of this kind of enzyme for plastic degradation. It is suggested
that further studies are needed to confirm the effectiveness of the enzymes. The study highlights
the importance of continuing to explore biological solutions for plastic waste management and
the challenges that come with developing new solutions in this field. (Less)
Please use this url to cite or link to this publication:
author
Hansen, Linus LU
supervisor
organization
course
KBTM05 20231
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Biotechnology, Enzymes, Plastic degradation, Plastic waste management
language
English
id
9125247
date added to LUP
2023-06-20 11:47:35
date last changed
2023-06-20 11:47:35
@misc{9125247,
  abstract     = {{In today's world, plastic pollution has become a widespread problem with plastics in oceans,
rivers and forests. The use of plastic has increased rapidly since it first was developed and that is
because of its advantageous abilities. This scientific report focuses on the evaluation of two
enzymes known as "autotransporter domains containing esterase" for its ability to degrade a
variety of plastics such as polyethylene terephthalate (PET). In this report the ability to degrade
plastics with enzymes has been tested by introducing two different autotransporter domains
containing esterase to different kinds of plastic and letting them react under different conditions
to determine the plastic degrading abilities in the enzymes. The results indicated that both
enzymes had an ability to degrade different plastics to some extent, but the extent of degradation
varied depending on the experimental conditions. However, the results were not entirely
conclusive as some of the results were contradictory. The biggest problem was replication of the
results, this can be due to several issues such as no expression of enzyme, problem with the
column and storage time of the enzyme. Despite the inconclusive results, the study provides
valuable insights into the potential of this kind of enzyme for plastic degradation. It is suggested
that further studies are needed to confirm the effectiveness of the enzymes. The study highlights
the importance of continuing to explore biological solutions for plastic waste management and
the challenges that come with developing new solutions in this field.}},
  author       = {{Hansen, Linus}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Assessment Of Plastic Degrading Abilities Of Autotransporter Domains Containing Esterase On Synthetic Polyesters}},
  year         = {{2023}},
}