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Chemoenzymatic Oxidation of polyolefins

Hassan Zadeh, Mina LU (2024) KBTM01 20241
Biotechnology (MSc)
Biotechnology (M.Sc.Eng.)
Abstract
The proliferation of plastic waste poses a significant contemporary challenge, driven by global population growth and the subsequent surge in plastic consumption. PE alone accounts for roughly 50-60% of the plastic waste stream. Its formidable resistance to enzymatic degradation, owing to its stable carbon-carbon (C-C) backbone and carbon-hydrogen (C-H) bonds, further exacerbates the issue. This study investigates the efficacy of pretreatment in initiating oxidation in PE films, alongside exploring enzymatic oxidation of Polyolefins using the Laccase enzyme. Findings reveal that a KMnO4/HCl pretreatment at 0.25/0.1 M, 60 °C for 24 hours induces carbonyl group (C=O) addition on the PE film surface. Following the treatment with Laccase Cot A... (More)
The proliferation of plastic waste poses a significant contemporary challenge, driven by global population growth and the subsequent surge in plastic consumption. PE alone accounts for roughly 50-60% of the plastic waste stream. Its formidable resistance to enzymatic degradation, owing to its stable carbon-carbon (C-C) backbone and carbon-hydrogen (C-H) bonds, further exacerbates the issue. This study investigates the efficacy of pretreatment in initiating oxidation in PE films, alongside exploring enzymatic oxidation of Polyolefins using the Laccase enzyme. Findings reveal that a KMnO4/HCl pretreatment at 0.25/0.1 M, 60 °C for 24 hours induces carbonyl group (C=O) addition on the PE film surface. Following the treatment with Laccase Cot A from Bacillus subtilis, in a TEMPO-mediated system (30 °C, 400 rpm), exhibits the capability to target PE films, introducing multiple functional groups (-OH, C=O, C=C, and C-O), indicative for the onset of oxidative degradation. Subsequent SEM analysis illustrates a notable increase in surface roughness, pore formation, and crack development post-enzymatic treatment of pretreated PE films. The study also notes accelerated oxidation and observable changes in PE films with an increase in enzyme concentration (from 1.02 µM to 4.25 µM). Additionally, Gas chromatography (GC) and High-performance liquid chromatography (HPLC) analyses of reaction solutions highlight the release of byproducts, signifying the initiation of PE film degradation. (Less)
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author
Hassan Zadeh, Mina LU
supervisor
organization
course
KBTM01 20241
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Plastic waste, Polyethylene (PE), Enzymatic degradation, Laccase enzyme, Oxidative degradation, biotechnology
language
English
id
9156655
date added to LUP
2024-06-11 08:25:44
date last changed
2024-06-11 08:25:44
@misc{9156655,
  abstract     = {{The proliferation of plastic waste poses a significant contemporary challenge, driven by global population growth and the subsequent surge in plastic consumption. PE alone accounts for roughly 50-60% of the plastic waste stream. Its formidable resistance to enzymatic degradation, owing to its stable carbon-carbon (C-C) backbone and carbon-hydrogen (C-H) bonds, further exacerbates the issue. This study investigates the efficacy of pretreatment in initiating oxidation in PE films, alongside exploring enzymatic oxidation of Polyolefins using the Laccase enzyme. Findings reveal that a KMnO4/HCl pretreatment at 0.25/0.1 M, 60 °C for 24 hours induces carbonyl group (C=O) addition on the PE film surface. Following the treatment with Laccase Cot A from Bacillus subtilis, in a TEMPO-mediated system (30 °C, 400 rpm), exhibits the capability to target PE films, introducing multiple functional groups (-OH, C=O, C=C, and C-O), indicative for the onset of oxidative degradation. Subsequent SEM analysis illustrates a notable increase in surface roughness, pore formation, and crack development post-enzymatic treatment of pretreated PE films. The study also notes accelerated oxidation and observable changes in PE films with an increase in enzyme concentration (from 1.02 µM to 4.25 µM). Additionally, Gas chromatography (GC) and High-performance liquid chromatography (HPLC) analyses of reaction solutions highlight the release of byproducts, signifying the initiation of PE film degradation.}},
  author       = {{Hassan Zadeh, Mina}},
  language     = {{eng}},
  note         = {{Student Paper}},
  title        = {{Chemoenzymatic Oxidation of polyolefins}},
  year         = {{2024}},
}