The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines.
Gjörloff Wingren, Anette LU ; Hadzic, Radinka LU ; Forsgren, Arne LU and Riesbeck, Kristian LU
(2002)
In Journal of Immunology
168(11).
p.5582-5588
- Abstract
- Moraxella IgD binding protein (MID) is a novel bacterial outer membrane protein with IgD-binding properties. MID was purified from the respiratory pathogen Moraxella catarrhalis and is here shown to have B cell stimulatory properties. Purified MID in the range of 0.01-0.1 microg/ml was optimal to induce a proliferative response in human PBL. MID coupled to Sepharose and formalin-fixed M. catarrhalis preparations induced similar proliferative responses in PBL cultures. MID or MID-Sepharose stimulated purified human peripheral B cells as measured by proliferation. In contrast, MID or MID-Sepharose did not activate T cells. Preincubation of purified B cells with anti-IgD Abs inhibited MID-Sepharose-induced B cell proliferation. The addition... (More)
- Moraxella IgD binding protein (MID) is a novel bacterial outer membrane protein with IgD-binding properties. MID was purified from the respiratory pathogen Moraxella catarrhalis and is here shown to have B cell stimulatory properties. Purified MID in the range of 0.01-0.1 microg/ml was optimal to induce a proliferative response in human PBL. MID coupled to Sepharose and formalin-fixed M. catarrhalis preparations induced similar proliferative responses in PBL cultures. MID or MID-Sepharose stimulated purified human peripheral B cells as measured by proliferation. In contrast, MID or MID-Sepharose did not activate T cells. Preincubation of purified B cells with anti-IgD Abs inhibited MID-Sepharose-induced B cell proliferation. The addition of IL-4 specifically induced IL-6 production in MID-Sepharose-activated B cells. IgM secretion was detected in B cell cultures stimulated with MID or MID-Sepharose and IL-2 for 10 days. Secretion of IgG and IgA was efficiently induced in cultures from purified B cells stimulated with the combination of MID or MID-Sepharose and IL-4, IL-10, and soluble CD40 ligand, suggesting that Th2-derived cytokines were required for optimal plasma cell generation. Taken together, MID has properties that make it an important tool to study IgD-targeted activation of B cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/108357
- author
- Gjörloff Wingren, Anette
LU
; Hadzic, Radinka
LU
; Forsgren, Arne
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Immunoglobulin D : physiology, Immunoglobulin A : biosynthesis, Human, Drug, Dose-Response Relationship, Cytokines, Carrier Proteins : pharmacology, B-Lymphocytes : immunology, B-Lymphocytes : drug effects, Interleukin-6 : biosynthesis, Lymphocyte Transformation : drug effects, Support, Non-U.S. Gov't, Th2 Cells : immunology, Immunoglobulin G : biosynthesis, Immunoglobulin M : biosynthesis, Interleukin-2 : pharmacology
- in
- Journal of Immunology
- volume
- 168
- issue
- 11
- pages
- 5582 - 5588
- publisher
- American Association of Immunologists
- external identifiers
-
- wos:000175815200026
- pmid:12023354
- scopus:0036604206
- ISSN
- 1550-6606
- language
- English
- LU publication?
- yes
- id
- 0e476d19-7e97-4ec9-91a2-562bcf1b93e2 (old id 108357)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12023354&dopt=Abstract
- date added to LUP
- 2016-04-01 16:30:01
- date last changed
- 2022-04-15 05:01:04
@article{0e476d19-7e97-4ec9-91a2-562bcf1b93e2,
abstract = {{Moraxella IgD binding protein (MID) is a novel bacterial outer membrane protein with IgD-binding properties. MID was purified from the respiratory pathogen Moraxella catarrhalis and is here shown to have B cell stimulatory properties. Purified MID in the range of 0.01-0.1 microg/ml was optimal to induce a proliferative response in human PBL. MID coupled to Sepharose and formalin-fixed M. catarrhalis preparations induced similar proliferative responses in PBL cultures. MID or MID-Sepharose stimulated purified human peripheral B cells as measured by proliferation. In contrast, MID or MID-Sepharose did not activate T cells. Preincubation of purified B cells with anti-IgD Abs inhibited MID-Sepharose-induced B cell proliferation. The addition of IL-4 specifically induced IL-6 production in MID-Sepharose-activated B cells. IgM secretion was detected in B cell cultures stimulated with MID or MID-Sepharose and IL-2 for 10 days. Secretion of IgG and IgA was efficiently induced in cultures from purified B cells stimulated with the combination of MID or MID-Sepharose and IL-4, IL-10, and soluble CD40 ligand, suggesting that Th2-derived cytokines were required for optimal plasma cell generation. Taken together, MID has properties that make it an important tool to study IgD-targeted activation of B cells.}},
author = {{Gjörloff Wingren, Anette and Hadzic, Radinka and Forsgren, Arne and Riesbeck, Kristian}},
issn = {{1550-6606}},
keywords = {{Immunoglobulin D : physiology; Immunoglobulin A : biosynthesis; Human; Drug; Dose-Response Relationship; Cytokines; Carrier Proteins : pharmacology; B-Lymphocytes : immunology; B-Lymphocytes : drug effects; Interleukin-6 : biosynthesis; Lymphocyte Transformation : drug effects; Support; Non-U.S. Gov't; Th2 Cells : immunology; Immunoglobulin G : biosynthesis; Immunoglobulin M : biosynthesis; Interleukin-2 : pharmacology}},
language = {{eng}},
number = {{11}},
pages = {{5582--5588}},
publisher = {{American Association of Immunologists}},
series = {{Journal of Immunology}},
title = {{The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines.}},
url = {{http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12023354&dopt=Abstract}},
volume = {{168}},
year = {{2002}},
}