Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture
(1993) In Experimental Brain Research 92(3). p.516-523- Abstract
- The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated... (More)
- The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated cultures of human but not rat tissue the TH-positive neurons had longer neurites than observed in control or PDGF-AA-treated cultures. These data indicate that PDGF-BB may act as a trophic factor for mesencephalic dopaminergic neurons and suggest that administration of PDGF-BB could ameliorate degeneration and possibly promote axonal sprouting of these neurons in vivo. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107589
- author
- Nikkhah, G ; Odin, Per LU ; Smits, A ; Tingström, Anders LU ; Sanmartin, Agneta LU ; Brundin, Patrik LU ; Funa, K and Lindvall, Olle LU
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Dopaminergic neurons, Platelet-derived growth factor, Cell culture, Parkinson's disease, Rat, Human
- in
- Experimental Brain Research
- volume
- 92
- issue
- 3
- pages
- 516 - 523
- publisher
- Springer
- external identifiers
-
- pmid:8095907
- scopus:0027082893
- ISSN
- 0014-4819
- DOI
- 10.1007/BF00229041
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neurology, Lund (013027000), Molecular Psychiatry Unit (013024100), Neuronal Survival (013212041)
- id
- b965dfa5-eda2-4ffc-814e-69b1fe285368 (old id 1107589)
- date added to LUP
- 2016-04-01 11:50:54
- date last changed
- 2021-01-03 06:41:58
@article{b965dfa5-eda2-4ffc-814e-69b1fe285368, abstract = {{The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated cultures of human but not rat tissue the TH-positive neurons had longer neurites than observed in control or PDGF-AA-treated cultures. These data indicate that PDGF-BB may act as a trophic factor for mesencephalic dopaminergic neurons and suggest that administration of PDGF-BB could ameliorate degeneration and possibly promote axonal sprouting of these neurons in vivo.}}, author = {{Nikkhah, G and Odin, Per and Smits, A and Tingström, Anders and Sanmartin, Agneta and Brundin, Patrik and Funa, K and Lindvall, Olle}}, issn = {{0014-4819}}, keywords = {{Dopaminergic neurons; Platelet-derived growth factor; Cell culture; Parkinson's disease; Rat; Human}}, language = {{eng}}, number = {{3}}, pages = {{516--523}}, publisher = {{Springer}}, series = {{Experimental Brain Research}}, title = {{Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture}}, url = {{http://dx.doi.org/10.1007/BF00229041}}, doi = {{10.1007/BF00229041}}, volume = {{92}}, year = {{1993}}, }