Mechanisms of muscarinic receptor-stimulated expression of c-fos in SH-SY5Y cells
(1994) In European Journal of Pharmacology 268(1). p.19-28- Abstract
- In this study, the signal cascade transducing carbachol stimulation into c-fos expression in SH-SY5Y neuroblastoma cells was investigated. 1,2-Diacylglycerol formation and c-fos expression were mediated via stimulation of muscarinic M1 receptors and the first 5 min of receptor stimulation were critical for these events. Application of 1,2-dioctanoylglycerol induced c-fos expression and this, as well as carbachol-stimulated c-fos expression, was inhibited by protein kinase C inhibitors. Increasing the intracellular Ca2+ concentration had only small effects on c-fos expression. There was a dependency on extracellular Ca2+ for maximal c-fos expression and 1,2-diacylglycerol formation. The carbachol-stimulated c-fos expression was potentiated... (More)
- In this study, the signal cascade transducing carbachol stimulation into c-fos expression in SH-SY5Y neuroblastoma cells was investigated. 1,2-Diacylglycerol formation and c-fos expression were mediated via stimulation of muscarinic M1 receptors and the first 5 min of receptor stimulation were critical for these events. Application of 1,2-dioctanoylglycerol induced c-fos expression and this, as well as carbachol-stimulated c-fos expression, was inhibited by protein kinase C inhibitors. Increasing the intracellular Ca2+ concentration had only small effects on c-fos expression. There was a dependency on extracellular Ca2+ for maximal c-fos expression and 1,2-diacylglycerol formation. The carbachol-stimulated c-fos expression was potentiated by application of the protein phosphatase inhibitor okadaic acid. These results demonstrate the importance of 1,2-diacylglycerol formation for muscarinic receptor-stimulated, protein kinase C-mediated c-fos expression in the SH-SY5Y cells and that this cascade is counteracted by an okadaic acid-sensitive protein phosphatase. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107715
- author
- Larsson, Christer LU ; Gustavsson, L ; Simonsson, Per LU ; Bergman, O and Alling, Christer LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Pharmacology
- volume
- 268
- issue
- 1
- pages
- 19 - 28
- publisher
- Elsevier
- external identifiers
-
- pmid:7925609
- scopus:43949150957
- ISSN
- 1879-0712
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530), Clinical Chemistry, Malmö (013016000), Division of Clinical Chemistry and Pharmacology (013250300)
- id
- e8bce303-919f-4d93-a44b-1be22716a2da (old id 1107715)
- date added to LUP
- 2016-04-01 12:02:14
- date last changed
- 2021-12-27 05:59:33
@article{e8bce303-919f-4d93-a44b-1be22716a2da, abstract = {{In this study, the signal cascade transducing carbachol stimulation into c-fos expression in SH-SY5Y neuroblastoma cells was investigated. 1,2-Diacylglycerol formation and c-fos expression were mediated via stimulation of muscarinic M1 receptors and the first 5 min of receptor stimulation were critical for these events. Application of 1,2-dioctanoylglycerol induced c-fos expression and this, as well as carbachol-stimulated c-fos expression, was inhibited by protein kinase C inhibitors. Increasing the intracellular Ca2+ concentration had only small effects on c-fos expression. There was a dependency on extracellular Ca2+ for maximal c-fos expression and 1,2-diacylglycerol formation. The carbachol-stimulated c-fos expression was potentiated by application of the protein phosphatase inhibitor okadaic acid. These results demonstrate the importance of 1,2-diacylglycerol formation for muscarinic receptor-stimulated, protein kinase C-mediated c-fos expression in the SH-SY5Y cells and that this cascade is counteracted by an okadaic acid-sensitive protein phosphatase.}}, author = {{Larsson, Christer and Gustavsson, L and Simonsson, Per and Bergman, O and Alling, Christer}}, issn = {{1879-0712}}, language = {{eng}}, number = {{1}}, pages = {{19--28}}, publisher = {{Elsevier}}, series = {{European Journal of Pharmacology}}, title = {{Mechanisms of muscarinic receptor-stimulated expression of c-fos in SH-SY5Y cells}}, volume = {{268}}, year = {{1994}}, }