Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells
(1997) In Brain Research. Molecular Brain Research 46(1-2). p.77-84- Abstract
- The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol... (More)
- The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111179
- author
- Ding, W Q ; Larsson, Christer LU ; Simonsson, Per LU and Alling, Christer LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Muscarinic acetylcholine receptor, Ethanol, c-fos expression, Protein kinase C, Neuroblastoma SH-SY5Y cell
- in
- Brain Research. Molecular Brain Research
- volume
- 46
- issue
- 1-2
- pages
- 77 - 84
- publisher
- Elsevier
- external identifiers
-
- pmid:9191080
- scopus:0030911415
- ISSN
- 0169-328X
- DOI
- 10.1016/S0169-328X(96)00273-2
- language
- English
- LU publication?
- yes
- id
- 962d2d5d-8b3d-4304-a616-596ad4d14622 (old id 1111179)
- date added to LUP
- 2016-04-01 16:01:12
- date last changed
- 2022-01-28 08:44:20
@article{962d2d5d-8b3d-4304-a616-596ad4d14622, abstract = {{The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure.}}, author = {{Ding, W Q and Larsson, Christer and Simonsson, Per and Alling, Christer}}, issn = {{0169-328X}}, keywords = {{Muscarinic acetylcholine receptor; Ethanol; c-fos expression; Protein kinase C; Neuroblastoma SH-SY5Y cell}}, language = {{eng}}, number = {{1-2}}, pages = {{77--84}}, publisher = {{Elsevier}}, series = {{Brain Research. Molecular Brain Research}}, title = {{Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells}}, url = {{http://dx.doi.org/10.1016/S0169-328X(96)00273-2}}, doi = {{10.1016/S0169-328X(96)00273-2}}, volume = {{46}}, year = {{1997}}, }