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Ethanol exposure potentiates fosB and junB expression induced by muscarinic receptor stimulation in neuroblastoma SH-SY5Y cells

Ding, Wei-Qun ; Fried, Ulrik LU ; Larsson, Christer LU and Alling, Christer LU (1998) In Alcoholism: Clinical and Experimental Research 22(1). p.225-230
Abstract
Muscarinic receptor stimulation and activation of protein kinase C cause an increase in fosB and junB transcripts in human neuroblastoma SH-SY5Y cells. In this study, the effect of long-term ethanol exposure on these events was investigated. Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. The potentiation was time- and dose-dependent on ethanol. Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Long-term ethanol exposure did not influence the... (More)
Muscarinic receptor stimulation and activation of protein kinase C cause an increase in fosB and junB transcripts in human neuroblastoma SH-SY5Y cells. In this study, the effect of long-term ethanol exposure on these events was investigated. Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. The potentiation was time- and dose-dependent on ethanol. Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Long-term ethanol exposure did not influence the expression of fosB and junB induced by activation of protein kinase C with phorbol ester. These results demonstrate that the muscarinic receptor-stimulated fosB and junB expression is sensitive to ethanol exposure in SH-SY5Y cells, suggesting that these genes participate in the regulation of neuronal function in response to chronic ethanol treatment. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ethanol, fosBJunB, Muscarinic Receptor, Protein Kinase C
in
Alcoholism: Clinical and Experimental Research
volume
22
issue
1
pages
225 - 230
publisher
Wiley-Blackwell
external identifiers
  • pmid:9514311
  • scopus:0031884921
ISSN
0145-6008
DOI
10.1111/j.1530-0277.1998.tb03642.x
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530), Division of Clinical Chemistry and Pharmacology (013250300)
id
8c0e93aa-9456-4738-97a7-41ecf90631d9 (old id 1112633)
date added to LUP
2016-04-01 11:37:17
date last changed
2022-02-25 18:56:35
@article{8c0e93aa-9456-4738-97a7-41ecf90631d9,
  abstract     = {{Muscarinic receptor stimulation and activation of protein kinase C cause an increase in fosB and junB transcripts in human neuroblastoma SH-SY5Y cells. In this study, the effect of long-term ethanol exposure on these events was investigated. Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. The potentiation was time- and dose-dependent on ethanol. Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Long-term ethanol exposure did not influence the expression of fosB and junB induced by activation of protein kinase C with phorbol ester. These results demonstrate that the muscarinic receptor-stimulated fosB and junB expression is sensitive to ethanol exposure in SH-SY5Y cells, suggesting that these genes participate in the regulation of neuronal function in response to chronic ethanol treatment.}},
  author       = {{Ding, Wei-Qun and Fried, Ulrik and Larsson, Christer and Alling, Christer}},
  issn         = {{0145-6008}},
  keywords     = {{Ethanol; fosBJunB; Muscarinic Receptor; Protein Kinase C}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{225--230}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Alcoholism: Clinical and Experimental Research}},
  title        = {{Ethanol exposure potentiates fosB and junB expression induced by muscarinic receptor stimulation in neuroblastoma SH-SY5Y cells}},
  url          = {{http://dx.doi.org/10.1111/j.1530-0277.1998.tb03642.x}},
  doi          = {{10.1111/j.1530-0277.1998.tb03642.x}},
  volume       = {{22}},
  year         = {{1998}},
}