Human achaete-scute homologue 1 (HASH-1) is downregulated in differentiating neuroblastoma cells
(1999) In Biochemical and Biophysical Research Communications 256(3). p.557-563- Abstract
- The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of... (More)
- The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents. It is concluded that moderate HASH-1 expression does not compromise the capacity of these cells to differentiate. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1114121
- author
- Söderholm, H ; Örtoft, Eva ; Johansson, Irja ; Ljungberg, June LU ; Larsson, Christer LU ; Axelson, Håkan LU and Påhlman, Sven LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- 256
- issue
- 3
- pages
- 557 - 563
- publisher
- Elsevier
- external identifiers
-
- pmid:10080936
- scopus:0342668639
- pmid:10080936
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.1999.0314
- language
- English
- LU publication?
- yes
- id
- f4f404ff-4c1a-41d8-a794-b5c5d4fc4ff2 (old id 1114121)
- date added to LUP
- 2016-04-01 16:43:28
- date last changed
- 2024-01-26 12:22:22
@article{f4f404ff-4c1a-41d8-a794-b5c5d4fc4ff2, abstract = {{The mammalian achaete-scute homologue, MASH-1, is crucial for early development of the sympathetic nervous system and is transiently expressed in sympathetic neuroblasts during embryogenesis. Here we report that the human homologue (HASH-1) was expressed in all analyzed cell lines (6/6) derived from the sympathetic nervous system tumor neuroblastoma. The majority of small-cell lung carcinoma (4/5) cell lines tested expressed HASH-1, while other nonneuronal/non-neuroendocrine cell lines were negative. Induced differentiation of neuroblastoma cells resulted in HASH-1 downregulation. This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents. It is concluded that moderate HASH-1 expression does not compromise the capacity of these cells to differentiate.}}, author = {{Söderholm, H and Örtoft, Eva and Johansson, Irja and Ljungberg, June and Larsson, Christer and Axelson, Håkan and Påhlman, Sven}}, issn = {{1090-2104}}, language = {{eng}}, number = {{3}}, pages = {{557--563}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Human achaete-scute homologue 1 (HASH-1) is downregulated in differentiating neuroblastoma cells}}, url = {{http://dx.doi.org/10.1006/bbrc.1999.0314}}, doi = {{10.1006/bbrc.1999.0314}}, volume = {{256}}, year = {{1999}}, }