Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit
(1999) In Acta Crystallographica. Section D: Biological Crystallography D55. p.689-690- Abstract
- The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 Å. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 Å.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/125452
- author
- Willows, R D ; Hansson, Mats LU ; Beale, S I ; Laurberg, Martin LU and Al-Karadaghi, Salam LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bacteriochlorophyll., chlorophyll, magnesium chelatase BchI subunit
- in
- Acta Crystallographica. Section D: Biological Crystallography
- volume
- D55
- pages
- 689 - 690
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:13044280254
- ISSN
- 1399-0047
- DOI
- 10.1107/S0907444998014759
- language
- English
- LU publication?
- yes
- id
- f974d91d-7759-44b6-a1a9-1a4b3b33e368 (old id 125452)
- date added to LUP
- 2016-04-01 16:57:32
- date last changed
- 2022-01-28 23:22:51
@article{f974d91d-7759-44b6-a1a9-1a4b3b33e368, abstract = {{The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 Å. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 Å.}}, author = {{Willows, R D and Hansson, Mats and Beale, S I and Laurberg, Martin and Al-Karadaghi, Salam}}, issn = {{1399-0047}}, keywords = {{bacteriochlorophyll.; chlorophyll; magnesium chelatase BchI subunit}}, language = {{eng}}, pages = {{689--690}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Acta Crystallographica. Section D: Biological Crystallography}}, title = {{Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit}}, url = {{http://dx.doi.org/10.1107/S0907444998014759}}, doi = {{10.1107/S0907444998014759}}, volume = {{D55}}, year = {{1999}}, }