Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography
Mölder, Anna ; Sebesta, Mikael ; Gustafsson, Mats LU
; Gisselson, Lennart
; Gjörloff Wingren, Anette
and Alm, Kersti
LU
(2008)
In Journal of Microscopy
232(2).
p.240-247
- Abstract
- Manual cell counting is time consuming and requires a high
degree of skill on behalf of the person performing the count.
Here we use a technique that utilizes digital holography,
allowing label-free and completely non-invasive cell counting
directly in cell culture vessels with adherent viable cells.
The images produced can provide both quantitative and
qualitative phase information from a single hologram.
The recently constructed microscope HolomonitorTM (Phase
Holographic Imaging AB, Lund, Sweden) combines the
commonly used phase contrast microscope with digital
holography, the latter giving us the possibility of achieving
quantitative... (More) - Manual cell counting is time consuming and requires a high
degree of skill on behalf of the person performing the count.
Here we use a technique that utilizes digital holography,
allowing label-free and completely non-invasive cell counting
directly in cell culture vessels with adherent viable cells.
The images produced can provide both quantitative and
qualitative phase information from a single hologram.
The recently constructed microscope HolomonitorTM (Phase
Holographic Imaging AB, Lund, Sweden) combines the
commonly used phase contrast microscope with digital
holography, the latter giving us the possibility of achieving
quantitative information on cellular shape, area, confluence
and optical thickness. This project aimed at determining the
accuracy and repeatability of cell counting measurements
using digital holography compared to the conventional
manual cell counting method using a haemocytometer. The
collected datawere also used to determine cell size and cellular
optical thickness.Theresults showthat digital holography can
be used for non-invasive automatic cell counting as precisely
as conventional manual cell counting (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1270751
- author
- Mölder, Anna
; Sebesta, Mikael
; Gustafsson, Mats
LU
; Gisselson, Lennart
; Gjörloff Wingren, Anette
and Alm, Kersti
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell analysis, cellular optical thickness, cell size, cell counting, digital holography
- in
- Journal of Microscopy
- volume
- 232
- issue
- 2
- pages
- 240 - 247
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000260544500007
- scopus:55449137792
- ISSN
- 0022-2720
- DOI
- 10.1111/j.1365-2818.2008.02095.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Electrical and information technology (011041010), Department of Cell and Organism Biology (Closed 2011.) (011002100)
- id
- 0b0c4c3b-0d2b-49ab-a214-94aa3c30c22f (old id 1270751)
- date added to LUP
- 2016-04-01 11:49:07
- date last changed
- 2022-03-28 03:31:57
@article{0b0c4c3b-0d2b-49ab-a214-94aa3c30c22f,
abstract = {{Manual cell counting is time consuming and requires a high<br/><br>
degree of skill on behalf of the person performing the count.<br/><br>
Here we use a technique that utilizes digital holography,<br/><br>
allowing label-free and completely non-invasive cell counting<br/><br>
directly in cell culture vessels with adherent viable cells.<br/><br>
The images produced can provide both quantitative and<br/><br>
qualitative phase information from a single hologram.<br/><br>
The recently constructed microscope HolomonitorTM (Phase<br/><br>
Holographic Imaging AB, Lund, Sweden) combines the<br/><br>
commonly used phase contrast microscope with digital<br/><br>
holography, the latter giving us the possibility of achieving<br/><br>
quantitative information on cellular shape, area, confluence<br/><br>
and optical thickness. This project aimed at determining the<br/><br>
accuracy and repeatability of cell counting measurements<br/><br>
using digital holography compared to the conventional<br/><br>
manual cell counting method using a haemocytometer. The<br/><br>
collected datawere also used to determine cell size and cellular<br/><br>
optical thickness.Theresults showthat digital holography can<br/><br>
be used for non-invasive automatic cell counting as precisely<br/><br>
as conventional manual cell counting}},
author = {{Mölder, Anna and Sebesta, Mikael and Gustafsson, Mats and Gisselson, Lennart and Gjörloff Wingren, Anette and Alm, Kersti}},
issn = {{0022-2720}},
keywords = {{Cell analysis; cellular optical thickness; cell size; cell counting; digital holography}},
language = {{eng}},
number = {{2}},
pages = {{240--247}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Microscopy}},
title = {{Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography}},
url = {{http://dx.doi.org/10.1111/j.1365-2818.2008.02095.x}},
doi = {{10.1111/j.1365-2818.2008.02095.x}},
volume = {{232}},
year = {{2008}},
}