CRIM1 is localized to the podocyte filtration slit diaphragm of the adult human kidney
Nyström, Jenny ; Hultenby, Kjell ; Ek, Sara LU ; Sjölund, Jonas LU ; Axelson, Håkan LU ; Jirström, Karin LU
; Saleem, Moin
; Nilsson, Kristina
LU
and Johansson, Martin
LU
(2009)
In Nephrology Dialysis Transplantation
24(7).
p.2038-2044
- Abstract
- Background. CRIM1 is a plasma membrane bound protein
containing six cysteine-rich repeats (CRR). Through these,
CRIM1 has been shown to interact with a subgroup of the
TGF-β superfamily, the bone morphogenic proteins (BMP)
isoforms 2, 4 and 7. The probable action is to modulate
the signalling properties of these factors. CRIM1 has also
been shown to regulate the release of VEGFA by podocytes
during renal organogenesis. Knock-out studies in mice have
shown that CRIM1 is critically involved in the development
of the central nervous system, eye and kidney. Replacement
of CRIM1 with a defective version leads to renal dysgenesis
and perinatal... (More) - Background. CRIM1 is a plasma membrane bound protein
containing six cysteine-rich repeats (CRR). Through these,
CRIM1 has been shown to interact with a subgroup of the
TGF-β superfamily, the bone morphogenic proteins (BMP)
isoforms 2, 4 and 7. The probable action is to modulate
the signalling properties of these factors. CRIM1 has also
been shown to regulate the release of VEGFA by podocytes
during renal organogenesis. Knock-out studies in mice have
shown that CRIM1 is critically involved in the development
of the central nervous system, eye and kidney. Replacement
of CRIM1 with a defective version leads to renal dysgenesis
and perinatal death. We have analysed the distribution of
CRIM1 in adult human renal tissue.
Methods. To this end, we have used immunofluorescence,
immunohistochemistry and immunoelectron microscopy.
We performed western blotting for the CRIM1 protein,
using lysates from isolated glomerular podocytes and human
renal tissue homogenate. By using quantitative PCR,
we compared the CRIM1 mRNA levels in podocytes, human
renal tissue homogenate, primary human renal proximal
tubular epithelial cells and primary human pulmonary
artery smooth muscle cells.
Results. The results show that in the human adult kidney,
CRIM1 is mainly expressed in the glomerular podocytes
and is associated with the insertional region of the filtration
slit diaphragm (SD) of the podocyte pedicles.
Conclusions. CRIM1 is a protein that should be added to
the list of proteins associated with the podocyte filtration
SD and with the probable action of modulating BMP and
VEGFA signalling. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1288636
- author
- Nyström, Jenny
; Hultenby, Kjell
; Ek, Sara
LU
; Sjölund, Jonas
LU
; Axelson, Håkan
LU
; Jirström, Karin
LU
; Saleem, Moin
; Nilsson, Kristina
LU
and Johansson, Martin
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- podocyte, immunoelectron microscopy, bone morphogenic protein, filtration slit membrane
- in
- Nephrology Dialysis Transplantation
- volume
- 24
- issue
- 7
- pages
- 2038 - 2044
- publisher
- Oxford University Press
- external identifiers
-
- wos:000267226500009
- pmid:19158190
- scopus:67651121922
- pmid:19158190
- ISSN
- 1460-2385
- DOI
- 10.1093/ndt/gfn743
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Molecular Tumour Biology (013017540), Pathology (Malmö) (013031000), Department of Immunotechnology (011029300), Pathology, (Lund) (013030000) Department affilation moved from v1000583 (Molecular Tumour Biology) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:41:48.
- id
- 2b2194b6-312f-418f-adcc-fd1a9d95beda (old id 1288636)
- date added to LUP
- 2016-04-01 15:05:38
- date last changed
- 2024-01-29 02:53:28
@article{2b2194b6-312f-418f-adcc-fd1a9d95beda,
abstract = {{Background. CRIM1 is a plasma membrane bound protein<br/><br>
containing six cysteine-rich repeats (CRR). Through these,<br/><br>
CRIM1 has been shown to interact with a subgroup of the<br/><br>
TGF-β superfamily, the bone morphogenic proteins (BMP)<br/><br>
isoforms 2, 4 and 7. The probable action is to modulate<br/><br>
the signalling properties of these factors. CRIM1 has also<br/><br>
been shown to regulate the release of VEGFA by podocytes<br/><br>
during renal organogenesis. Knock-out studies in mice have<br/><br>
shown that CRIM1 is critically involved in the development<br/><br>
of the central nervous system, eye and kidney. Replacement<br/><br>
of CRIM1 with a defective version leads to renal dysgenesis<br/><br>
and perinatal death. We have analysed the distribution of<br/><br>
CRIM1 in adult human renal tissue.<br/><br>
Methods. To this end, we have used immunofluorescence,<br/><br>
immunohistochemistry and immunoelectron microscopy.<br/><br>
We performed western blotting for the CRIM1 protein,<br/><br>
using lysates from isolated glomerular podocytes and human<br/><br>
renal tissue homogenate. By using quantitative PCR,<br/><br>
we compared the CRIM1 mRNA levels in podocytes, human<br/><br>
renal tissue homogenate, primary human renal proximal<br/><br>
tubular epithelial cells and primary human pulmonary<br/><br>
artery smooth muscle cells.<br/><br>
Results. The results show that in the human adult kidney,<br/><br>
CRIM1 is mainly expressed in the glomerular podocytes<br/><br>
and is associated with the insertional region of the filtration<br/><br>
slit diaphragm (SD) of the podocyte pedicles.<br/><br>
Conclusions. CRIM1 is a protein that should be added to<br/><br>
the list of proteins associated with the podocyte filtration<br/><br>
SD and with the probable action of modulating BMP and<br/><br>
VEGFA signalling.}},
author = {{Nyström, Jenny and Hultenby, Kjell and Ek, Sara and Sjölund, Jonas and Axelson, Håkan and Jirström, Karin and Saleem, Moin and Nilsson, Kristina and Johansson, Martin}},
issn = {{1460-2385}},
keywords = {{podocyte; immunoelectron microscopy; bone morphogenic protein; filtration slit membrane}},
language = {{eng}},
number = {{7}},
pages = {{2038--2044}},
publisher = {{Oxford University Press}},
series = {{Nephrology Dialysis Transplantation}},
title = {{CRIM1 is localized to the podocyte filtration slit diaphragm of the adult human kidney}},
url = {{http://dx.doi.org/10.1093/ndt/gfn743}},
doi = {{10.1093/ndt/gfn743}},
volume = {{24}},
year = {{2009}},
}