Effects of ultraviolet-radiation on viability of isolated beta-vulgaris and hordeum-vulgare protoplasts
(1982) In Zeitschrift für Pflanzenphysiologie 105(4). p.297-306- Abstract
- Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B... (More)
- Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B radiation decreased the intensity of DLE by 40% without appreciably affecting the decay rate. Longer treatment times did not give a proportional effect so that even after 60 min of UV-B the inhibition did not exceed 60%. This suggested that although the enzyme system responsible for FDA hydrolysis may be partially inactivated (viability was 75-80% as compared with 90% in the control), the UV-B did not penetrate the innermost parts of the chloroplasts, but left some thylakoids undamaged. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/134623
- author
- Bornman, Janet LU ; Bornman, C H and Björn, Lars Olof LU
- organization
- publishing date
- 1982
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Zeitschrift für Pflanzenphysiologie
- volume
- 105
- issue
- 4
- pages
- 297 - 306
- publisher
- Gustav Fischer Verlag
- ISSN
- 0044-328X
- language
- English
- LU publication?
- yes
- id
- 5bf3b6c7-efb6-4f12-bc66-2b5480bf4563 (old id 134623)
- date added to LUP
- 2016-04-01 15:54:29
- date last changed
- 2018-11-21 20:37:17
@article{5bf3b6c7-efb6-4f12-bc66-2b5480bf4563, abstract = {{Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B radiation decreased the intensity of DLE by 40% without appreciably affecting the decay rate. Longer treatment times did not give a proportional effect so that even after 60 min of UV-B the inhibition did not exceed 60%. This suggested that although the enzyme system responsible for FDA hydrolysis may be partially inactivated (viability was 75-80% as compared with 90% in the control), the UV-B did not penetrate the innermost parts of the chloroplasts, but left some thylakoids undamaged.}}, author = {{Bornman, Janet and Bornman, C H and Björn, Lars Olof}}, issn = {{0044-328X}}, language = {{eng}}, number = {{4}}, pages = {{297--306}}, publisher = {{Gustav Fischer Verlag}}, series = {{Zeitschrift für Pflanzenphysiologie}}, title = {{Effects of ultraviolet-radiation on viability of isolated beta-vulgaris and hordeum-vulgare protoplasts}}, volume = {{105}}, year = {{1982}}, }