Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Lipopolysaccharide induces cell death in cultured porcine myenteric neurons.

Arciszewski, Marcin ; Pierzynowski, Stefan LU and Ekblad, Eva LU (2005) In Digestive Diseases and Sciences 50(9). p.1661-1668
Abstract
Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous... (More)
Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous presence of α-ketoglutarate or L-NAME. Either SNAP or α-ketoglutarate influenced neuronal survival. Culturing, particularly in the presence of LPS, markedly increased the proportion of VIP-immunoreactive neurons; NOS-immunoreactive neurons were unchanged. The reported LPS-induced neurotoxicity indicates loss of enteric neurons as a consequence of intestinal inflammation. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Myenteric Plexus: cytology, Animals, Cell Culture Techniques, Cell Death, Cell Survival, Female, Immunohistochemistry, Inflammation, Intestinal Diseases: etiology, Intestinal Diseases: immunology, Intestinal Diseases: microbiology, Lipopolysaccharides: pharmacology, Male, Myenteric Plexus: pathology, Neurons: drug effects, Neurons: physiology, Neurotransmitters: biosynthesis, Research Support, Non-U.S. Gov't, Swine
in
Digestive Diseases and Sciences
volume
50
issue
9
pages
1661 - 1668
publisher
Springer
external identifiers
  • wos:000231312700015
  • pmid:16133966
  • scopus:23944452404
  • pmid:16133966
ISSN
1573-2568
DOI
10.1007/s10620-005-2912-2
language
English
LU publication?
yes
id
fa83cbee-9c50-43a1-92d6-e36db1bada1d (old id 143918)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16133966&dopt=Abstract
date added to LUP
2016-04-01 11:59:10
date last changed
2022-01-26 21:11:40
@article{fa83cbee-9c50-43a1-92d6-e36db1bada1d,
  abstract     = {{Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous presence of α-ketoglutarate or L-NAME. Either SNAP or α-ketoglutarate influenced neuronal survival. Culturing, particularly in the presence of LPS, markedly increased the proportion of VIP-immunoreactive neurons; NOS-immunoreactive neurons were unchanged. The reported LPS-induced neurotoxicity indicates loss of enteric neurons as a consequence of intestinal inflammation.}},
  author       = {{Arciszewski, Marcin and Pierzynowski, Stefan and Ekblad, Eva}},
  issn         = {{1573-2568}},
  keywords     = {{Myenteric Plexus: cytology; Animals; Cell Culture Techniques; Cell Death; Cell Survival; Female; Immunohistochemistry; Inflammation; Intestinal Diseases: etiology; Intestinal Diseases: immunology; Intestinal Diseases: microbiology; Lipopolysaccharides: pharmacology; Male; Myenteric Plexus: pathology; Neurons: drug effects; Neurons: physiology; Neurotransmitters: biosynthesis; Research Support; Non-U.S. Gov't; Swine}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1661--1668}},
  publisher    = {{Springer}},
  series       = {{Digestive Diseases and Sciences}},
  title        = {{Lipopolysaccharide induces cell death in cultured porcine myenteric neurons.}},
  url          = {{https://lup.lub.lu.se/search/files/2731467/624981.pdf}},
  doi          = {{10.1007/s10620-005-2912-2}},
  volume       = {{50}},
  year         = {{2005}},
}