Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells
(2009) In Nucleosides, Nucleotides & Nucleic Acids 28(11-12). p.1068-1075- Abstract
- Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1531546
- author
- Hebrard, Claire ; Cros-Perrial, Emeline ; Clausen, Anders Ranegaard LU ; Dumontet, Charles ; Piskur, Jure LU and Jordheim, Lars Petter
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bacteria, suicide gene, gene therapy, Deoxyribonucleoside kinases, gemcitabine, cancer, resistance
- in
- Nucleosides, Nucleotides & Nucleic Acids
- volume
- 28
- issue
- 11-12
- pages
- 1068 - 1075
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000273107100008
- scopus:77949633741
- pmid:20183574
- ISSN
- 1525-7770
- DOI
- 10.1080/15257770903368393
- language
- English
- LU publication?
- yes
- id
- 0b124203-7d16-46ab-bf05-47f9a4a6d254 (old id 1531546)
- date added to LUP
- 2016-04-01 12:04:35
- date last changed
- 2022-01-26 22:29:33
@article{0b124203-7d16-46ab-bf05-47f9a4a6d254, abstract = {{Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.}}, author = {{Hebrard, Claire and Cros-Perrial, Emeline and Clausen, Anders Ranegaard and Dumontet, Charles and Piskur, Jure and Jordheim, Lars Petter}}, issn = {{1525-7770}}, keywords = {{bacteria; suicide gene; gene therapy; Deoxyribonucleoside kinases; gemcitabine; cancer; resistance}}, language = {{eng}}, number = {{11-12}}, pages = {{1068--1075}}, publisher = {{Taylor & Francis}}, series = {{Nucleosides, Nucleotides & Nucleic Acids}}, title = {{Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells}}, url = {{http://dx.doi.org/10.1080/15257770903368393}}, doi = {{10.1080/15257770903368393}}, volume = {{28}}, year = {{2009}}, }