Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2
(2005) In Journal of Agricultural and Food Chemistry 53(17). p.6658-6665- Abstract
- Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length... (More)
- Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/153233
- author
- Janicke, Birgit LU ; Önning, Gunilla LU and Oredsson, S M
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- p-coumaric acid, Cell cycle, proliferation, cereals, ferulic acid
- in
- Journal of Agricultural and Food Chemistry
- volume
- 53
- issue
- 17
- pages
- 6658 - 6665
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:16104781
- wos:000231459900015
- scopus:24344464239
- pmid:16104781
- ISSN
- 0021-8561
- DOI
- 10.1021/jf050489l
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pure and Applied Biochemistry (LTH) (011001005), Animal Physiology (Closed 2011) (011011000), Center for Chemistry and Chemical Engineering (011001000)
- id
- b4b7ef2f-f837-4f3c-82d3-3176515208f6 (old id 153233)
- date added to LUP
- 2016-04-01 11:46:45
- date last changed
- 2022-03-20 18:49:55
@article{b4b7ef2f-f837-4f3c-82d3-3176515208f6, abstract = {{Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer.}}, author = {{Janicke, Birgit and Önning, Gunilla and Oredsson, S M}}, issn = {{0021-8561}}, keywords = {{p-coumaric acid; Cell cycle; proliferation; cereals; ferulic acid}}, language = {{eng}}, number = {{17}}, pages = {{6658--6665}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Agricultural and Food Chemistry}}, title = {{Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2}}, url = {{http://dx.doi.org/10.1021/jf050489l}}, doi = {{10.1021/jf050489l}}, volume = {{53}}, year = {{2005}}, }