Characterization of Lewy body pathology in 12- and 16-year-old intrastriatal mesencephalic grafts surviving in a patient with Parkinson's disease.
(2010) In Movement Disorders 25(8). p.1091-1096- Abstract
- We previously reported the occurrence of Lewy bodies in grafted human fetal mesencephalic neurons in two patients with Parkinson's disease. Here, we have used immunohistochemistry and electron microscopy to characterize the development of Lewy bodies in one of these cases. This patient was operated in putamen on both sides at 12 or 16 years before death, respectively. We demonstrate that 2% of the 12-year-old and 5% of the 16-year-old grafted, presumed dopaminergic neurons contained Lewy bodies immunoreactive for alpha-synuclein. Based on morphological analysis, two forms of alpha-synuclein-positive aggregates were distinguished in the grafts, the first a classical and compact Lewy body, the other a loose meshwork aggregate. Lewy bodies in... (More)
- We previously reported the occurrence of Lewy bodies in grafted human fetal mesencephalic neurons in two patients with Parkinson's disease. Here, we have used immunohistochemistry and electron microscopy to characterize the development of Lewy bodies in one of these cases. This patient was operated in putamen on both sides at 12 or 16 years before death, respectively. We demonstrate that 2% of the 12-year-old and 5% of the 16-year-old grafted, presumed dopaminergic neurons contained Lewy bodies immunoreactive for alpha-synuclein. Based on morphological analysis, two forms of alpha-synuclein-positive aggregates were distinguished in the grafts, the first a classical and compact Lewy body, the other a loose meshwork aggregate. Lewy bodies in the grafts stained positively for ubiquitin and thioflavin-S, and contained characteristic alpha-synuclein immunoreactive electron dense fibrillar structures on electron microscopy. Our data indicate that Lewy bodies develop gradually in transplanted dopaminergic neurons in a fashion similar to that in dopaminergic neurons in the host substantia nigra. (c) 2010 Movement Disorder Society. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1582610
- author
- Li, Jia-Yi LU ; Englund, Elisabet LU ; Widner, Håkan LU ; Rehncrona, Stig LU ; Björklund, Anders LU ; Lindvall, Olle LU and Brundin, Patrik LU
- organization
-
- Neural Plasticity and Repair (research group)
- Tumor microenvironment
- Neurology, Lund
- Neurosurgery
- Neurobiology (research group)
- Department of Experimental Medical Science
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- neural transplantation, transmissible neurological disease, Lewy body, alpha-synuclein, protein aggregation
- in
- Movement Disorders
- volume
- 25
- issue
- 8
- pages
- 1091 - 1096
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000279044600019
- pmid:20198645
- scopus:77949820437
- pmid:20198645
- ISSN
- 0885-3185
- DOI
- 10.1002/mds.23012
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041), Neurobiology (013212024), Pathology, (Lund) (013030000), Neurology, Lund (013027000), Neurosurgery (013026000), Neural Plasticity and Repair (013210080)
- id
- 40867eef-c7f9-449b-8e0e-5a0a56c75550 (old id 1582610)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20198645?dopt=Abstract
- date added to LUP
- 2016-04-01 10:37:12
- date last changed
- 2022-05-18 00:33:12
@article{40867eef-c7f9-449b-8e0e-5a0a56c75550, abstract = {{We previously reported the occurrence of Lewy bodies in grafted human fetal mesencephalic neurons in two patients with Parkinson's disease. Here, we have used immunohistochemistry and electron microscopy to characterize the development of Lewy bodies in one of these cases. This patient was operated in putamen on both sides at 12 or 16 years before death, respectively. We demonstrate that 2% of the 12-year-old and 5% of the 16-year-old grafted, presumed dopaminergic neurons contained Lewy bodies immunoreactive for alpha-synuclein. Based on morphological analysis, two forms of alpha-synuclein-positive aggregates were distinguished in the grafts, the first a classical and compact Lewy body, the other a loose meshwork aggregate. Lewy bodies in the grafts stained positively for ubiquitin and thioflavin-S, and contained characteristic alpha-synuclein immunoreactive electron dense fibrillar structures on electron microscopy. Our data indicate that Lewy bodies develop gradually in transplanted dopaminergic neurons in a fashion similar to that in dopaminergic neurons in the host substantia nigra. (c) 2010 Movement Disorder Society.}}, author = {{Li, Jia-Yi and Englund, Elisabet and Widner, Håkan and Rehncrona, Stig and Björklund, Anders and Lindvall, Olle and Brundin, Patrik}}, issn = {{0885-3185}}, keywords = {{neural transplantation; transmissible neurological disease; Lewy body; alpha-synuclein; protein aggregation}}, language = {{eng}}, number = {{8}}, pages = {{1091--1096}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Movement Disorders}}, title = {{Characterization of Lewy body pathology in 12- and 16-year-old intrastriatal mesencephalic grafts surviving in a patient with Parkinson's disease.}}, url = {{http://dx.doi.org/10.1002/mds.23012}}, doi = {{10.1002/mds.23012}}, volume = {{25}}, year = {{2010}}, }