Small Islets are Essential for Successful Intraportal Transplantation in a Diabetes Mouse Model
(2010) In Scandinavian Journal of Immunology 72(6). p.504-510- Abstract
- Optimization of islet transplantation protocols is necessary for improved success of treatment for type 1 diabetes. Here, we investigated whether the size of islets transplanted into the portal vein (PV) of the liver can affect engraftment in the early post-transplantation in an experimental mouse model. Small (average diameter < 250 mu m, group A) or large (average diameter > 250 mu m, group B) islets (400 islet equivalents/recipient) purified from normal BALB/c mice were transplanted into syngenic recipients with diabetes induced by STZ. The percentage of mice returning to a non-diabetic status was higher in group A (100%) than that of group B (62.5%). Focal areas of liver necrosis associated with the islets emboli were observed in... (More)
- Optimization of islet transplantation protocols is necessary for improved success of treatment for type 1 diabetes. Here, we investigated whether the size of islets transplanted into the portal vein (PV) of the liver can affect engraftment in the early post-transplantation in an experimental mouse model. Small (average diameter < 250 mu m, group A) or large (average diameter > 250 mu m, group B) islets (400 islet equivalents/recipient) purified from normal BALB/c mice were transplanted into syngenic recipients with diabetes induced by STZ. The percentage of mice returning to a non-diabetic status was higher in group A (100%) than that of group B (62.5%). Focal areas of liver necrosis associated with the islets emboli were observed in both groups, but the pathology in group B was significantly worse. Multiple proinflammatory cytokines were significantly higher in group B than that of A at 3 h post-transplantation. Our study determined that the size of islets plays a critical role in the success of intraportal islet transplantation (IPIT) and should be taken into account in future IPIT protocols for the treatment of diabetes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1752312
- author
- Su, Z. ; Xia, J. ; Shao, W. ; Cui, Y. ; Tai, S. ; Ekberg, Henrik LU ; Corbascio, M. ; Chen, J. and Qi, Z.
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Immunology
- volume
- 72
- issue
- 6
- pages
- 504 - 510
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000283993100004
- scopus:78149257689
- pmid:21044124
- ISSN
- 1365-3083
- DOI
- 10.1111/j.1365-3083.2010.02466.x
- language
- English
- LU publication?
- yes
- id
- 04b2fde5-fa06-4ad0-a098-16443609739f (old id 1752312)
- date added to LUP
- 2016-04-01 14:10:15
- date last changed
- 2022-01-27 23:06:54
@article{04b2fde5-fa06-4ad0-a098-16443609739f, abstract = {{Optimization of islet transplantation protocols is necessary for improved success of treatment for type 1 diabetes. Here, we investigated whether the size of islets transplanted into the portal vein (PV) of the liver can affect engraftment in the early post-transplantation in an experimental mouse model. Small (average diameter < 250 mu m, group A) or large (average diameter > 250 mu m, group B) islets (400 islet equivalents/recipient) purified from normal BALB/c mice were transplanted into syngenic recipients with diabetes induced by STZ. The percentage of mice returning to a non-diabetic status was higher in group A (100%) than that of group B (62.5%). Focal areas of liver necrosis associated with the islets emboli were observed in both groups, but the pathology in group B was significantly worse. Multiple proinflammatory cytokines were significantly higher in group B than that of A at 3 h post-transplantation. Our study determined that the size of islets plays a critical role in the success of intraportal islet transplantation (IPIT) and should be taken into account in future IPIT protocols for the treatment of diabetes.}}, author = {{Su, Z. and Xia, J. and Shao, W. and Cui, Y. and Tai, S. and Ekberg, Henrik and Corbascio, M. and Chen, J. and Qi, Z.}}, issn = {{1365-3083}}, language = {{eng}}, number = {{6}}, pages = {{504--510}}, publisher = {{Wiley-Blackwell}}, series = {{Scandinavian Journal of Immunology}}, title = {{Small Islets are Essential for Successful Intraportal Transplantation in a Diabetes Mouse Model}}, url = {{http://dx.doi.org/10.1111/j.1365-3083.2010.02466.x}}, doi = {{10.1111/j.1365-3083.2010.02466.x}}, volume = {{72}}, year = {{2010}}, }