Pseudomonas aeruginosa in cystic fibrosis: Pyocyanin negative strains are associated with BPI-ANCA and progressive lung disease.
Carlsson, Malin ; Shukla, Swati LU ; Petersson, Ann Cathrine ; Segelmark, Mårten LU and Hellmark, Thomas LU
(2011)
In Journal of Cystic Fibrosis
10.
p.265-271
- Abstract
- The clinical consequence of chronic Pseudomonas aeruginosa colonization in cystic fibrosis (CF) varies between individuals for unknown reasons. Auto-antibodies against bactericidal/permeability increasing protein (BPI-ANCA) are associated with poor prognosis in CF. We hypothesize that there is a correlation between the presence of BPI-ANCA, the properties of the colonizing bacteria and the clinical conditions of the host. We compared isolates of P. aeruginosa from BPI-ANCA positive CF patients who have deteriorating lung disease with BPI-ANCA negative CF patients who are in stable clinical conditions. Epithelial cells (A549) and isolated polymorphonuclear granulocytes (PMNs) were stimulated with the isolates and cell death was analyzed... (More)
- The clinical consequence of chronic Pseudomonas aeruginosa colonization in cystic fibrosis (CF) varies between individuals for unknown reasons. Auto-antibodies against bactericidal/permeability increasing protein (BPI-ANCA) are associated with poor prognosis in CF. We hypothesize that there is a correlation between the presence of BPI-ANCA, the properties of the colonizing bacteria and the clinical conditions of the host. We compared isolates of P. aeruginosa from BPI-ANCA positive CF patients who have deteriorating lung disease with BPI-ANCA negative CF patients who are in stable clinical conditions. Epithelial cells (A549) and isolated polymorphonuclear granulocytes (PMNs) were stimulated with the isolates and cell death was analyzed with flow cytometry. We found that the ANCA associated strains in most cases showed pyocyanin negative phenotypes. These strains also induced less inflammatory response than the non-ANCA associated strains as shown by apoptosis and necrosis of epithelial cells and neutrophils. Our results suggest that colonization with strains of P. aeruginosa that induce a weak inflammatory response is associated with unfavorable outcome in CF. We speculate that inadequate control of pathogen proliferation through an insufficient inflammatory response results in a slowly increasing number of bacteria and accumulation of dying PMNs in the airways, contributing to progression in CF lung disease. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1937517
- author
- Carlsson, Malin
; Shukla, Swati
LU
; Petersson, Ann Cathrine
; Segelmark, Mårten
LU
and Hellmark, Thomas
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Cystic Fibrosis
- volume
- 10
- pages
- 265 - 271
- publisher
- Elsevier
- external identifiers
-
- wos:000292065900007
- pmid:21463973
- scopus:79958252832
- pmid:21463973
- ISSN
- 1873-5010
- DOI
- 10.1016/j.jcf.2011.03.004
- language
- English
- LU publication?
- yes
- id
- e79554db-72c7-4a0c-a87d-0fa5d3e126b1 (old id 1937517)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21463973?dopt=Abstract
- date added to LUP
- 2016-04-04 08:52:36
- date last changed
- 2022-04-15 20:56:49
@article{e79554db-72c7-4a0c-a87d-0fa5d3e126b1,
abstract = {{The clinical consequence of chronic Pseudomonas aeruginosa colonization in cystic fibrosis (CF) varies between individuals for unknown reasons. Auto-antibodies against bactericidal/permeability increasing protein (BPI-ANCA) are associated with poor prognosis in CF. We hypothesize that there is a correlation between the presence of BPI-ANCA, the properties of the colonizing bacteria and the clinical conditions of the host. We compared isolates of P. aeruginosa from BPI-ANCA positive CF patients who have deteriorating lung disease with BPI-ANCA negative CF patients who are in stable clinical conditions. Epithelial cells (A549) and isolated polymorphonuclear granulocytes (PMNs) were stimulated with the isolates and cell death was analyzed with flow cytometry. We found that the ANCA associated strains in most cases showed pyocyanin negative phenotypes. These strains also induced less inflammatory response than the non-ANCA associated strains as shown by apoptosis and necrosis of epithelial cells and neutrophils. Our results suggest that colonization with strains of P. aeruginosa that induce a weak inflammatory response is associated with unfavorable outcome in CF. We speculate that inadequate control of pathogen proliferation through an insufficient inflammatory response results in a slowly increasing number of bacteria and accumulation of dying PMNs in the airways, contributing to progression in CF lung disease.}},
author = {{Carlsson, Malin and Shukla, Swati and Petersson, Ann Cathrine and Segelmark, Mårten and Hellmark, Thomas}},
issn = {{1873-5010}},
language = {{eng}},
pages = {{265--271}},
publisher = {{Elsevier}},
series = {{Journal of Cystic Fibrosis}},
title = {{Pseudomonas aeruginosa in cystic fibrosis: Pyocyanin negative strains are associated with BPI-ANCA and progressive lung disease.}},
url = {{http://dx.doi.org/10.1016/j.jcf.2011.03.004}},
doi = {{10.1016/j.jcf.2011.03.004}},
volume = {{10}},
year = {{2011}},
}