Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.
(2011) In Journal of Infectious Diseases 203(8). p.1096-1100- Abstract
- To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high... (More)
- To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high MCV load (P < .01). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1937699
- author
- Faust, Helena LU ; Pastrana, Diana V ; Buck, Christopher B ; Dillner, Joakim LU and Ekström, Johanna LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 203
- issue
- 8
- pages
- 1096 - 1100
- publisher
- Oxford University Press
- external identifiers
-
- wos:000289168500009
- pmid:21450999
- scopus:79953320829
- pmid:21450999
- ISSN
- 1537-6613
- DOI
- 10.1093/infdis/jiq173
- language
- English
- LU publication?
- yes
- id
- bd5fad1f-6dc4-4cb0-b421-3f7e9bba54a7 (old id 1937699)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21450999?dopt=Abstract
- date added to LUP
- 2016-04-01 13:06:30
- date last changed
- 2022-01-27 17:23:08
@article{bd5fad1f-6dc4-4cb0-b421-3f7e9bba54a7, abstract = {{To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high MCV load (P < .01).}}, author = {{Faust, Helena and Pastrana, Diana V and Buck, Christopher B and Dillner, Joakim and Ekström, Johanna}}, issn = {{1537-6613}}, language = {{eng}}, number = {{8}}, pages = {{1096--1100}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.}}, url = {{https://lup.lub.lu.se/search/files/3163988/1971350.pdf}}, doi = {{10.1093/infdis/jiq173}}, volume = {{203}}, year = {{2011}}, }