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Characterisation of a glycosylated alkyl polyglycoside produced by a cyclodextrin glycosyltransferase by HPLC-ELSD and -MS.

Svensson, David LU and Adlercreutz, Patrick LU orcid (2011) In Journal of Chromatography. B 879. p.1857-1860
Abstract
A transglycosylation reaction between an alkyl polyglycoside and α-cyclodextrin catalysed by cyclodextrin glycosyltransferase (CGTase) from Bacillus macerans was investigated. The reaction products were identified by comparison with standards generated by CGTase catalysed modification of pure alkyl glycosides using HPLC-ELSD and -MS analysis. The main products were alkyl glucopyranosides (substrates present in the alkyl polyglycoside) glycosylated with 6 (primary coupling products) or 12 (secondary coupling products) glucose residues. Both α and β anomers were glycosylated.
Please use this url to cite or link to this publication:
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type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography. B
volume
879
pages
1857 - 1860
publisher
Elsevier
external identifiers
  • wos:000292665600003
  • pmid:21621486
  • scopus:80955177600
  • pmid:21621486
ISSN
1873-376X
DOI
10.1016/j.jchromb.2011.04.037
language
English
LU publication?
yes
id
0a6fd34b-f63a-439c-bfb2-8204bb1803a9 (old id 1971961)
date added to LUP
2016-04-01 10:45:33
date last changed
2022-01-26 02:15:22
@article{0a6fd34b-f63a-439c-bfb2-8204bb1803a9,
  abstract     = {{A transglycosylation reaction between an alkyl polyglycoside and α-cyclodextrin catalysed by cyclodextrin glycosyltransferase (CGTase) from Bacillus macerans was investigated. The reaction products were identified by comparison with standards generated by CGTase catalysed modification of pure alkyl glycosides using HPLC-ELSD and -MS analysis. The main products were alkyl glucopyranosides (substrates present in the alkyl polyglycoside) glycosylated with 6 (primary coupling products) or 12 (secondary coupling products) glucose residues. Both α and β anomers were glycosylated.}},
  author       = {{Svensson, David and Adlercreutz, Patrick}},
  issn         = {{1873-376X}},
  language     = {{eng}},
  pages        = {{1857--1860}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography. B}},
  title        = {{Characterisation of a glycosylated alkyl polyglycoside produced by a cyclodextrin glycosyltransferase by HPLC-ELSD and -MS.}},
  url          = {{http://dx.doi.org/10.1016/j.jchromb.2011.04.037}},
  doi          = {{10.1016/j.jchromb.2011.04.037}},
  volume       = {{879}},
  year         = {{2011}},
}