Regeneration in, and properties of, extracted peripheral nerve allografts and xenografts.
Kvist, Martin LU ; Sondell, Mariann ; Kanje, Martin and Dahlin, Lars LU
(2011)
In Journal of Plastic Surgery and Hand Surgery
45(3).
p.122-128
- Abstract
- Abstract When not enough conventional autologous nerve grafts are available, alternatives are needed to bridge nerve defects. Our aim was to study regeneration of nerves in chemically-extracted acellular nerve grafts from frogs, mice, humans (fresh and stored sural nerve), pigs and rats when defects in rat sciatic nerves were bridged. Secondly, we compared two different extraction procedures (techniques described by Sondell et al. and Hudson et al.) with respect to how efficiently they supported axonal outgrowth, and remaining laminin and myelin basic protein (MBP), after extraction. Isografts (rat) and xenografts (mouse) were transplanted into defects in rat sciatic nerves. Acellular nerve allografts from rats, extracted by the Sondell et... (More)
- Abstract When not enough conventional autologous nerve grafts are available, alternatives are needed to bridge nerve defects. Our aim was to study regeneration of nerves in chemically-extracted acellular nerve grafts from frogs, mice, humans (fresh and stored sural nerve), pigs and rats when defects in rat sciatic nerves were bridged. Secondly, we compared two different extraction procedures (techniques described by Sondell et al. and Hudson et al.) with respect to how efficiently they supported axonal outgrowth, and remaining laminin and myelin basic protein (MBP), after extraction. Isografts (rat) and xenografts (mouse) were transplanted into defects in rat sciatic nerves. Acellular nerve allografts from rats, extracted by the Sondell et al's technique, had an appreciably longer axonal outgrowth based on immunohistochemical staining of neurofilaments, than acellular nerve xenografts except those from the pig. Among acellular xenografts there was considerably longer axonal outgrowth in the grafts from pigs compared with those from humans (fresh), but there were no other differences among the xenografts with respect to axonal outgrowth. Axonal outgrowth in acellular nerve xenografts from mice, extracted by the method described by Sondell et al. was longer than in those extracted by Hudson et al's method, while there was no difference in outgrowth between extracted nerve isografts from rats. Electrophoretic analysis of extracted acellular nerve grafts showed remaining laminin, but not MBP, after both extraction procedures. These preserved laminin and removed MBP in acellular nerve grafts. Such grafts can be used to reconstruct short defects in nerves irrespective of their origin. However, selecting and matching a suitable combination of graft and host species may improve axonal outgrowth. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2007946
- author
- Kvist, Martin
LU
; Sondell, Mariann
; Kanje, Martin
and Dahlin, Lars
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Plastic Surgery and Hand Surgery
- volume
- 45
- issue
- 3
- pages
- 122 - 128
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000291815000001
- pmid:21682608
- scopus:79959407377
- pmid:21682608
- ISSN
- 2000-656X
- DOI
- 10.3109/2000656X.2011.571847
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Reconstructive Surgery (013240300), Hand Surgery Research Group (013241910)
- id
- 52c185e3-ac79-427b-bb5a-f1c102145df4 (old id 2007946)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21682608?dopt=Abstract
- date added to LUP
- 2016-04-01 11:01:06
- date last changed
- 2022-04-12 19:53:36
@article{52c185e3-ac79-427b-bb5a-f1c102145df4,
abstract = {{Abstract When not enough conventional autologous nerve grafts are available, alternatives are needed to bridge nerve defects. Our aim was to study regeneration of nerves in chemically-extracted acellular nerve grafts from frogs, mice, humans (fresh and stored sural nerve), pigs and rats when defects in rat sciatic nerves were bridged. Secondly, we compared two different extraction procedures (techniques described by Sondell et al. and Hudson et al.) with respect to how efficiently they supported axonal outgrowth, and remaining laminin and myelin basic protein (MBP), after extraction. Isografts (rat) and xenografts (mouse) were transplanted into defects in rat sciatic nerves. Acellular nerve allografts from rats, extracted by the Sondell et al's technique, had an appreciably longer axonal outgrowth based on immunohistochemical staining of neurofilaments, than acellular nerve xenografts except those from the pig. Among acellular xenografts there was considerably longer axonal outgrowth in the grafts from pigs compared with those from humans (fresh), but there were no other differences among the xenografts with respect to axonal outgrowth. Axonal outgrowth in acellular nerve xenografts from mice, extracted by the method described by Sondell et al. was longer than in those extracted by Hudson et al's method, while there was no difference in outgrowth between extracted nerve isografts from rats. Electrophoretic analysis of extracted acellular nerve grafts showed remaining laminin, but not MBP, after both extraction procedures. These preserved laminin and removed MBP in acellular nerve grafts. Such grafts can be used to reconstruct short defects in nerves irrespective of their origin. However, selecting and matching a suitable combination of graft and host species may improve axonal outgrowth.}},
author = {{Kvist, Martin and Sondell, Mariann and Kanje, Martin and Dahlin, Lars}},
issn = {{2000-656X}},
language = {{eng}},
number = {{3}},
pages = {{122--128}},
publisher = {{Taylor & Francis}},
series = {{Journal of Plastic Surgery and Hand Surgery}},
title = {{Regeneration in, and properties of, extracted peripheral nerve allografts and xenografts.}},
url = {{https://lup.lub.lu.se/search/files/2307228/2174105.pdf}},
doi = {{10.3109/2000656X.2011.571847}},
volume = {{45}},
year = {{2011}},
}