Coupling of permeabilized cells of Gluconobacter oxydans and Ralstonia eutropha for asymmetric ketone reduction using H(2) as reductant.
Rundbäck, Fabian LU ; Fidanoska, Milica LU and Adlercreutz, Patrick LU
(2012)
In Journal of Biotechnology
157.
p.154-158
- Abstract
- A combined two-cell reaction system containing Gluconobacter oxydans and Ralstonia eutropha was evaluated with regard to asymmetric ketone reduction using H(2) as the reductant. Whole cells permeabilized by EDTA/toluene were used, and synthesis was performed in a biphasic aqueous/organic reaction medium. The two-cell system was compared with a system in which G. oxydans alone was used for both ketone reduction and cofactor regeneration, using an alcohol as co-substrate. The two-cell system exhibited almost twice the initial reaction rate of the single-cell system, a higher yield (75% vs. 48%) but slightly lower enantiomeric purity (93% vs. 98%) of the product (S)-2-octanol. The permeabilized R. eutropha cells are worth evaluating for... (More)
- A combined two-cell reaction system containing Gluconobacter oxydans and Ralstonia eutropha was evaluated with regard to asymmetric ketone reduction using H(2) as the reductant. Whole cells permeabilized by EDTA/toluene were used, and synthesis was performed in a biphasic aqueous/organic reaction medium. The two-cell system was compared with a system in which G. oxydans alone was used for both ketone reduction and cofactor regeneration, using an alcohol as co-substrate. The two-cell system exhibited almost twice the initial reaction rate of the single-cell system, a higher yield (75% vs. 48%) but slightly lower enantiomeric purity (93% vs. 98%) of the product (S)-2-octanol. The permeabilized R. eutropha cells are worth evaluating for byproduct-free NADH regeneration in combination with other whole cell catalysts. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2200476
- author
- Rundbäck, Fabian
LU
; Fidanoska, Milica
LU
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biotechnology
- volume
- 157
- pages
- 154 - 158
- publisher
- Elsevier
- external identifiers
-
- wos:000298635200022
- pmid:22001848
- scopus:84855224161
- pmid:22001848
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2011.09.029
- language
- English
- LU publication?
- yes
- id
- 54d283fc-3ab7-4e34-b8ac-15a49ff9dc89 (old id 2200476)
- date added to LUP
- 2016-04-01 11:03:40
- date last changed
- 2022-01-26 05:05:10
@article{54d283fc-3ab7-4e34-b8ac-15a49ff9dc89,
abstract = {{A combined two-cell reaction system containing Gluconobacter oxydans and Ralstonia eutropha was evaluated with regard to asymmetric ketone reduction using H(2) as the reductant. Whole cells permeabilized by EDTA/toluene were used, and synthesis was performed in a biphasic aqueous/organic reaction medium. The two-cell system was compared with a system in which G. oxydans alone was used for both ketone reduction and cofactor regeneration, using an alcohol as co-substrate. The two-cell system exhibited almost twice the initial reaction rate of the single-cell system, a higher yield (75% vs. 48%) but slightly lower enantiomeric purity (93% vs. 98%) of the product (S)-2-octanol. The permeabilized R. eutropha cells are worth evaluating for byproduct-free NADH regeneration in combination with other whole cell catalysts.}},
author = {{Rundbäck, Fabian and Fidanoska, Milica and Adlercreutz, Patrick}},
issn = {{1873-4863}},
language = {{eng}},
pages = {{154--158}},
publisher = {{Elsevier}},
series = {{Journal of Biotechnology}},
title = {{Coupling of permeabilized cells of Gluconobacter oxydans and Ralstonia eutropha for asymmetric ketone reduction using H(2) as reductant.}},
url = {{http://dx.doi.org/10.1016/j.jbiotec.2011.09.029}},
doi = {{10.1016/j.jbiotec.2011.09.029}},
volume = {{157}},
year = {{2012}},
}