TraML: a standard format for exchange of selected reaction monitoring transition lists
(2012) In Molecular & Cellular Proteomics 11(4). p.111-015040- Abstract
- Abstract in Undetermined
Targeted proteomics via selected reaction monitoring (SRM) is a powerful mass spectrometric technique affording higher dynamic range, increased specificity and lower limits of detection than other shotgun mass spectrometry methods when applied to proteome analyses. However, it involves selective measurement of predetermined analytes, which requires more preparation in the form of selecting appropriate signatures for the proteins and peptides that are to be targeted. There is a growing number of software programs and resources for selecting optimal transitions and the instrument settings used for the detection and quantification of the targeted peptides, but the exchange of this information is hindered by a lack... (More) - Abstract in Undetermined
Targeted proteomics via selected reaction monitoring (SRM) is a powerful mass spectrometric technique affording higher dynamic range, increased specificity and lower limits of detection than other shotgun mass spectrometry methods when applied to proteome analyses. However, it involves selective measurement of predetermined analytes, which requires more preparation in the form of selecting appropriate signatures for the proteins and peptides that are to be targeted. There is a growing number of software programs and resources for selecting optimal transitions and the instrument settings used for the detection and quantification of the targeted peptides, but the exchange of this information is hindered by a lack of a standard format. We have developed a new standardized format, called TraML, for encoding transition lists and associated metadata. In addition to introducing the TraML format, we demonstrate several implementations across the community, and provide semantic validators, extensive documentation, and multiple example instances to demonstrate correctly written documents. Widespread use of TraML will facilitate the exchange of transitions, reduce time spent handling incompatible list formats, increase the reusability of previously optimized transitions, and thus accelerate the widespread adoption of targeted proteomics via SRM. (Less)
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https://lup.lub.lu.se/record/2225759
- author
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular & Cellular Proteomics
- volume
- 11
- issue
- 4
- pages
- 111 - 015040
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000302786500026
- scopus:84859843750
- ISSN
- 1535-9484
- DOI
- 10.1074/mcp.R111.015040
- language
- English
- LU publication?
- yes
- id
- 56a84c71-3224-4e58-942c-1219fe51aa4d (old id 2225759)
- date added to LUP
- 2016-04-01 11:08:59
- date last changed
- 2024-04-22 04:57:06
@article{56a84c71-3224-4e58-942c-1219fe51aa4d, abstract = {{Abstract in Undetermined<br/>Targeted proteomics via selected reaction monitoring (SRM) is a powerful mass spectrometric technique affording higher dynamic range, increased specificity and lower limits of detection than other shotgun mass spectrometry methods when applied to proteome analyses. However, it involves selective measurement of predetermined analytes, which requires more preparation in the form of selecting appropriate signatures for the proteins and peptides that are to be targeted. There is a growing number of software programs and resources for selecting optimal transitions and the instrument settings used for the detection and quantification of the targeted peptides, but the exchange of this information is hindered by a lack of a standard format. We have developed a new standardized format, called TraML, for encoding transition lists and associated metadata. In addition to introducing the TraML format, we demonstrate several implementations across the community, and provide semantic validators, extensive documentation, and multiple example instances to demonstrate correctly written documents. Widespread use of TraML will facilitate the exchange of transitions, reduce time spent handling incompatible list formats, increase the reusability of previously optimized transitions, and thus accelerate the widespread adoption of targeted proteomics via SRM.}}, author = {{Deutsch, Eric W. and Chambers, Matthew and Neumann, Steffen and Levander, Fredrik and Binz, Pierre-Alain and Shofstahl, Jim and Campbell, David S. and Mendoza, Luis and Ovelleiro, David and Helsens, Kenny and Martens, Lennart and Aebersold, Ruedi and Moritz, Robert L. and Brusniak, Mi-Youn}}, issn = {{1535-9484}}, language = {{eng}}, number = {{4}}, pages = {{111--015040}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Molecular & Cellular Proteomics}}, title = {{TraML: a standard format for exchange of selected reaction monitoring transition lists}}, url = {{http://dx.doi.org/10.1074/mcp.R111.015040}}, doi = {{10.1074/mcp.R111.015040}}, volume = {{11}}, year = {{2012}}, }