Oral and faecal lactobacilli and their expression of mannose-specific adhesins in individuals with and without IgA deficiency
(2012) In International Journal of Medical Microbiology 302(1). p.53-60- Abstract
- Lactobacilli are present in the intestine and oral cavity of most adults. Secretory IgA in mucosal secretions may provide carbohydrate receptors for bacterial adhesins. Here, oral and faecal samples from 33 IgA-deficient individuals and 34 controls were cultured for lactobacilli, which were identified using species-specific PCR or partial 165 rDNA sequencing and tested for expression of mannose-specific adhesins. Lactobacilli were found in the oral cavity of 76% of IgA-deficient and 85% of control individuals. Lactobacillus paracasei and Lactobacillus gasseri dominated in both groups. Lactobacillus fermentum was less common in IgA-deficient individuals than in controls (p = 0.0055) and Lactobacillus salivarius was less common in... (More)
- Lactobacilli are present in the intestine and oral cavity of most adults. Secretory IgA in mucosal secretions may provide carbohydrate receptors for bacterial adhesins. Here, oral and faecal samples from 33 IgA-deficient individuals and 34 controls were cultured for lactobacilli, which were identified using species-specific PCR or partial 165 rDNA sequencing and tested for expression of mannose-specific adhesins. Lactobacilli were found in the oral cavity of 76% of IgA-deficient and 85% of control individuals. Lactobacillus paracasei and Lactobacillus gasseri dominated in both groups. Lactobacillus fermentum was less common in IgA-deficient individuals than in controls (p = 0.0055) and Lactobacillus salivarius was less common in symptomatic than in healthy IgA-deficient individuals (p = 0.0051). Faecal samples yielded lactobacilli in most individuals. L. paracasei was most frequent, followed by L. gasseri and Lactobacillus plantarum. Mannose-specific adhesins were expressed more frequently by oral than by faecal isolates (p = 0.032) and oral isolates adhered in higher numbers than faecal isolates (46 vs. 14 bacteria/cell, p = 0.0038). Faecal isolates from IgA-deficient individuals more frequently expressed mannose-specific adhesins than faecal isolates from controls (p = 0.039). Mannose-specific adhesins may be a colonisation factor in the oral cavity, and the presence of secretory IgA may modify adhesin expression. However, secretory IgA seems to have little influence on Lactobacillus species distribution. (C) 2011 Elsevier GmbH. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2333899
- author
- Lonnermark, Elisabet ; Nowrouzinan, Forough ; Adlerberth, Ingegerd ; Ahrné, Siv LU ; Wold, Agnes and Friman, Vanda
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lactobacillus, IgA deficiency, Gut, Oral, Microbiota, Adhesion
- in
- International Journal of Medical Microbiology
- volume
- 302
- issue
- 1
- pages
- 53 - 60
- publisher
- Elsevier
- external identifiers
-
- wos:000298525500007
- scopus:81255157867
- ISSN
- 1618-0607
- DOI
- 10.1016/j.ijmm.2011.08.004
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300)
- id
- 162b01a9-f4ea-4613-ae94-815400f4c526 (old id 2333899)
- date added to LUP
- 2016-04-01 09:59:04
- date last changed
- 2023-10-11 14:51:16
@article{162b01a9-f4ea-4613-ae94-815400f4c526,
abstract = {{Lactobacilli are present in the intestine and oral cavity of most adults. Secretory IgA in mucosal secretions may provide carbohydrate receptors for bacterial adhesins. Here, oral and faecal samples from 33 IgA-deficient individuals and 34 controls were cultured for lactobacilli, which were identified using species-specific PCR or partial 165 rDNA sequencing and tested for expression of mannose-specific adhesins. Lactobacilli were found in the oral cavity of 76% of IgA-deficient and 85% of control individuals. Lactobacillus paracasei and Lactobacillus gasseri dominated in both groups. Lactobacillus fermentum was less common in IgA-deficient individuals than in controls (p = 0.0055) and Lactobacillus salivarius was less common in symptomatic than in healthy IgA-deficient individuals (p = 0.0051). Faecal samples yielded lactobacilli in most individuals. L. paracasei was most frequent, followed by L. gasseri and Lactobacillus plantarum. Mannose-specific adhesins were expressed more frequently by oral than by faecal isolates (p = 0.032) and oral isolates adhered in higher numbers than faecal isolates (46 vs. 14 bacteria/cell, p = 0.0038). Faecal isolates from IgA-deficient individuals more frequently expressed mannose-specific adhesins than faecal isolates from controls (p = 0.039). Mannose-specific adhesins may be a colonisation factor in the oral cavity, and the presence of secretory IgA may modify adhesin expression. However, secretory IgA seems to have little influence on Lactobacillus species distribution. (C) 2011 Elsevier GmbH. All rights reserved.}},
author = {{Lonnermark, Elisabet and Nowrouzinan, Forough and Adlerberth, Ingegerd and Ahrné, Siv and Wold, Agnes and Friman, Vanda}},
issn = {{1618-0607}},
keywords = {{Lactobacillus; IgA deficiency; Gut; Oral; Microbiota; Adhesion}},
language = {{eng}},
number = {{1}},
pages = {{53--60}},
publisher = {{Elsevier}},
series = {{International Journal of Medical Microbiology}},
title = {{Oral and faecal lactobacilli and their expression of mannose-specific adhesins in individuals with and without IgA deficiency}},
url = {{http://dx.doi.org/10.1016/j.ijmm.2011.08.004}},
doi = {{10.1016/j.ijmm.2011.08.004}},
volume = {{302}},
year = {{2012}},
}