Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry.
(2012) In Clinical Biochemistry 45(4-5). p.331-338- Abstract
- OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels.... (More)
- OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels. Multivariate analysis of clinical data revealed well-separated patient groups. CONCLUSIONS: We demonstrated that PSA in seminal plasma occurs in several isoforms, yet not all were detectable using an antibody based clinical routine method. The heterogeneity of PSA expression might be of clinical significance, by an improved patient phenotyping. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2336781
- author
- Végvári, Ákos LU ; Rezeli, Melinda LU ; Sihlbom, Carina ; Häkkinen, Jari LU ; Carlsohn, Elisabet ; Malm, Johan LU ; Lilja, Hans LU ; Laurell, Thomas LU and Marko-Varga, György LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ESI-LTQ FT-ICR, MALDI LTQ Orbitrap XL, infertility, seminal plasma, prostate specific antigen, isoform
- in
- Clinical Biochemistry
- volume
- 45
- issue
- 4-5
- pages
- 331 - 338
- publisher
- Elsevier
- external identifiers
-
- wos:000302844500012
- pmid:22209970
- scopus:84857655838
- pmid:22209970
- ISSN
- 1873-2933
- DOI
- 10.1016/j.clinbiochem.2011.11.018
- language
- English
- LU publication?
- yes
- id
- a84afa89-e40e-43fe-9a26-4aae21614c7a (old id 2336781)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22209970?dopt=Abstract
- date added to LUP
- 2016-04-01 10:14:52
- date last changed
- 2023-08-30 21:44:15
@article{a84afa89-e40e-43fe-9a26-4aae21614c7a, abstract = {{OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels. Multivariate analysis of clinical data revealed well-separated patient groups. CONCLUSIONS: We demonstrated that PSA in seminal plasma occurs in several isoforms, yet not all were detectable using an antibody based clinical routine method. The heterogeneity of PSA expression might be of clinical significance, by an improved patient phenotyping.}}, author = {{Végvári, Ákos and Rezeli, Melinda and Sihlbom, Carina and Häkkinen, Jari and Carlsohn, Elisabet and Malm, Johan and Lilja, Hans and Laurell, Thomas and Marko-Varga, György}}, issn = {{1873-2933}}, keywords = {{ESI-LTQ FT-ICR; MALDI LTQ Orbitrap XL; infertility; seminal plasma; prostate specific antigen; isoform}}, language = {{eng}}, number = {{4-5}}, pages = {{331--338}}, publisher = {{Elsevier}}, series = {{Clinical Biochemistry}}, title = {{Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry.}}, url = {{https://lup.lub.lu.se/search/files/1687369/2368812.pdf}}, doi = {{10.1016/j.clinbiochem.2011.11.018}}, volume = {{45}}, year = {{2012}}, }