Neuroblastoma and pre-B lymphoma cells share expression of key transcription factors but display tissue restricted target gene expression
(2004) In BMC Cancer 4.- Abstract
- Background: Transcription factors are frequently involved in the process of cellular transformation, and many malignancies are characterized by a distinct genetic event affecting a specific transcription factor. This probably reflects a tissue specific ability of transcription factors to contribute to the generation of cancer but very little is known about the precise mechanisms that governs these restricted effects. Methods: To investigate this selectivity in target gene activation we compared the overall gene expression patterns by micro-array analysis and expression of target genes for the transcription factor EBF in lymphoma and neuroblastoma cells by RT-PCR. The presence of transcription factors in the different model cell lines was... (More)
- Background: Transcription factors are frequently involved in the process of cellular transformation, and many malignancies are characterized by a distinct genetic event affecting a specific transcription factor. This probably reflects a tissue specific ability of transcription factors to contribute to the generation of cancer but very little is known about the precise mechanisms that governs these restricted effects. Methods: To investigate this selectivity in target gene activation we compared the overall gene expression patterns by micro-array analysis and expression of target genes for the transcription factor EBF in lymphoma and neuroblastoma cells by RT-PCR. The presence of transcription factors in the different model cell lines was further investigated by EMSA analysis. Results: In pre-B cells mb-1 and CD19 are regulate by EBF-1 in collaboration with Pax-5 and E-proteins. We here show that neuroblastoma cells express these three, for B cell development crucial transcription factors, but nevertheless fail to express detectable levels of their known target genes. Expression of mb-1 could, however, be induced in neuroblastoma cells after disruption of the chromatin structure by treatment with 5-azacytidine and Trichostatin A. Conclusion: These data suggest that transcription factors are able to selectively activate target genes in different tissues and that chromatin structure plays a key role in the regulation of this activity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/254957
- author
- Lagergren, Anna LU ; Manetopoulos, Christina LU ; Axelson, Håkan LU and Sigvardsson, Mikael LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- BMC Cancer
- volume
- 4
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:15544702
- wos:000226525400001
- scopus:13144291649
- ISSN
- 1471-2407
- DOI
- 10.1186/1471-2407-4-80
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Molecular Tumour Biology (013017540) Department affilation moved from v1000583 (Molecular Tumour Biology) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:41:48.
- id
- 04f52da9-ea47-4f15-9eac-9b7027cc237f (old id 254957)
- date added to LUP
- 2016-04-01 16:56:02
- date last changed
- 2022-01-28 23:12:01
@article{04f52da9-ea47-4f15-9eac-9b7027cc237f, abstract = {{Background: Transcription factors are frequently involved in the process of cellular transformation, and many malignancies are characterized by a distinct genetic event affecting a specific transcription factor. This probably reflects a tissue specific ability of transcription factors to contribute to the generation of cancer but very little is known about the precise mechanisms that governs these restricted effects. Methods: To investigate this selectivity in target gene activation we compared the overall gene expression patterns by micro-array analysis and expression of target genes for the transcription factor EBF in lymphoma and neuroblastoma cells by RT-PCR. The presence of transcription factors in the different model cell lines was further investigated by EMSA analysis. Results: In pre-B cells mb-1 and CD19 are regulate by EBF-1 in collaboration with Pax-5 and E-proteins. We here show that neuroblastoma cells express these three, for B cell development crucial transcription factors, but nevertheless fail to express detectable levels of their known target genes. Expression of mb-1 could, however, be induced in neuroblastoma cells after disruption of the chromatin structure by treatment with 5-azacytidine and Trichostatin A. Conclusion: These data suggest that transcription factors are able to selectively activate target genes in different tissues and that chromatin structure plays a key role in the regulation of this activity.}}, author = {{Lagergren, Anna and Manetopoulos, Christina and Axelson, Håkan and Sigvardsson, Mikael}}, issn = {{1471-2407}}, language = {{eng}}, publisher = {{BioMed Central (BMC)}}, series = {{BMC Cancer}}, title = {{Neuroblastoma and pre-B lymphoma cells share expression of key transcription factors but display tissue restricted target gene expression}}, url = {{http://dx.doi.org/10.1186/1471-2407-4-80}}, doi = {{10.1186/1471-2407-4-80}}, volume = {{4}}, year = {{2004}}, }