Initiation of the decorin glycosaminoglycan chain in the endoplasmic reticulum-Golgi intermediate compartment
(2003) In Journal of Biological Chemistry 278(24). p.21415-21420- Abstract
- We have transiently expressed decorin with a C- terminal KDEL endoplasmic reticulum retention signal peptide in COS- 7 cells to study initiation of galactosaminoglycan synthesis in the endoplasmic reticulum- Golgi intermediate compartment. All decorin- KDEL molecules were substituted with N- linked oligosaccharides sensitive to endoglycosidase H, indicating that the core protein was located proximal to the medial- Golgi. O-Linked glycosylation was only initiated in a minor fraction of the molecules. The O- linked saccharides were characterized by gel filtration after stepwise degradations using chondroitin ABC/ AC-I lyases, beta1 - 3- glycuronidase, beta-galactosidase, and alkaline phosphatase. The major O- linked saccharide was the... (More)
- We have transiently expressed decorin with a C- terminal KDEL endoplasmic reticulum retention signal peptide in COS- 7 cells to study initiation of galactosaminoglycan synthesis in the endoplasmic reticulum- Golgi intermediate compartment. All decorin- KDEL molecules were substituted with N- linked oligosaccharides sensitive to endoglycosidase H, indicating that the core protein was located proximal to the medial- Golgi. O-Linked glycosylation was only initiated in a minor fraction of the molecules. The O- linked saccharides were characterized by gel filtration after stepwise degradations using chondroitin ABC/ AC-I lyases, beta1 - 3- glycuronidase, beta-galactosidase, and alkaline phosphatase. The major O- linked saccharide was the linkage region pentasaccharide GalNAcbeta1-4GlcUAbeta1-3Galbeta1-3Galbeta1-4-Xyl- 2- phosphate, demonstrating initiation of chondroitin synthesis in the endoplasmic reticulum- Golgi intermediate compartment. In the presence of brefeldin A, partial elongation of a chondroitin chain took place, indicating retrieval of polymerases but not of sulfotransferases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/308889
- author
- Jönsson, Mats LU ; Eklund, Erik LU ; Fransson, Lars-Åke LU and Oldberg, Åke LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 278
- issue
- 24
- pages
- 21415 - 21420
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000183354200015
- scopus:0038498824
- pmid:12668676
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M210977200
- language
- English
- LU publication?
- yes
- id
- f422e802-d2a0-4c26-8eb8-5084f060b33e (old id 308889)
- date added to LUP
- 2016-04-01 12:05:27
- date last changed
- 2022-04-13 05:59:46
@article{f422e802-d2a0-4c26-8eb8-5084f060b33e, abstract = {{We have transiently expressed decorin with a C- terminal KDEL endoplasmic reticulum retention signal peptide in COS- 7 cells to study initiation of galactosaminoglycan synthesis in the endoplasmic reticulum- Golgi intermediate compartment. All decorin- KDEL molecules were substituted with N- linked oligosaccharides sensitive to endoglycosidase H, indicating that the core protein was located proximal to the medial- Golgi. O-Linked glycosylation was only initiated in a minor fraction of the molecules. The O- linked saccharides were characterized by gel filtration after stepwise degradations using chondroitin ABC/ AC-I lyases, beta1 - 3- glycuronidase, beta-galactosidase, and alkaline phosphatase. The major O- linked saccharide was the linkage region pentasaccharide GalNAcbeta1-4GlcUAbeta1-3Galbeta1-3Galbeta1-4-Xyl- 2- phosphate, demonstrating initiation of chondroitin synthesis in the endoplasmic reticulum- Golgi intermediate compartment. In the presence of brefeldin A, partial elongation of a chondroitin chain took place, indicating retrieval of polymerases but not of sulfotransferases.}}, author = {{Jönsson, Mats and Eklund, Erik and Fransson, Lars-Åke and Oldberg, Åke}}, issn = {{1083-351X}}, language = {{eng}}, number = {{24}}, pages = {{21415--21420}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{Initiation of the decorin glycosaminoglycan chain in the endoplasmic reticulum-Golgi intermediate compartment}}, url = {{http://dx.doi.org/10.1074/jbc.M210977200}}, doi = {{10.1074/jbc.M210977200}}, volume = {{278}}, year = {{2003}}, }