Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells
(2003) In EMBO Reports 4(4). p.400-404- Abstract
- Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-gradient centrifugation technique to separate culturable and non-culturable cells, we show that the proteins in non-culturable cells show increased and irreversible oxidative damage, which affects various bacterial compartments and proteins. The levels of expression of specific stress regulons are higher in non-culturable cells, confirming increased defects relating to oxidative damage and the occurrence of aberrant, such as by... (More)
- Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-gradient centrifugation technique to separate culturable and non-culturable cells, we show that the proteins in non-culturable cells show increased and irreversible oxidative damage, which affects various bacterial compartments and proteins. The levels of expression of specific stress regulons are higher in non-culturable cells, confirming increased defects relating to oxidative damage and the occurrence of aberrant, such as by amino-acid misincorporation, proteins. Our data suggest that non-culturable cells are produced due to stochastic deterioration, rather than an adaptive programme, and pinpoint oxidation management as the 'Achilles heel' of these cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/310964
- author
- Desnues, B ; Cuny, C ; Grégori, Gérald LU ; Dukan, S ; Aguilaniu, H and Nystrom, T
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- EMBO Reports
- volume
- 4
- issue
- 4
- pages
- 400 - 404
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:12671690
- wos:000182801300014
- scopus:0038779369
- ISSN
- 1469-221X
- DOI
- 10.1038/sj.embor.embor799
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Microbial Ecology (Closed 2011) (011008001)
- id
- 4fd8a07d-b4d6-47bb-8291-931f167f19e3 (old id 310964)
- date added to LUP
- 2016-04-01 16:58:41
- date last changed
- 2022-03-07 17:38:25
@article{4fd8a07d-b4d6-47bb-8291-931f167f19e3,
abstract = {{Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-gradient centrifugation technique to separate culturable and non-culturable cells, we show that the proteins in non-culturable cells show increased and irreversible oxidative damage, which affects various bacterial compartments and proteins. The levels of expression of specific stress regulons are higher in non-culturable cells, confirming increased defects relating to oxidative damage and the occurrence of aberrant, such as by amino-acid misincorporation, proteins. Our data suggest that non-culturable cells are produced due to stochastic deterioration, rather than an adaptive programme, and pinpoint oxidation management as the 'Achilles heel' of these cells.}},
author = {{Desnues, B and Cuny, C and Grégori, Gérald and Dukan, S and Aguilaniu, H and Nystrom, T}},
issn = {{1469-221X}},
language = {{eng}},
number = {{4}},
pages = {{400--404}},
publisher = {{Nature Publishing Group}},
series = {{EMBO Reports}},
title = {{Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells}},
url = {{http://dx.doi.org/10.1038/sj.embor.embor799}},
doi = {{10.1038/sj.embor.embor799}},
volume = {{4}},
year = {{2003}},
}