Rapid and direct determination of fructose in food: A new osmium-polymer mediated biosensor
(2013) In Food Chemistry 140(4). p.742-747- Abstract
- This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A... (More)
- This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A cm(-2) mM), good reproducibility (RSD = 2.1%) and a fast response time (4 s). Finally, the biosensor was applied for specific determination of fructose in honey, fruit juices, soft and energy drinks. The results indicated a very good agreement with those obtained with a commercial reference kit. No significant interference was observed with the proposed biosensor. (C) 2012 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3975344
- author
- Antiochia, Riccarda ; Vinci, Giuliana and Gorton, Lo LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fructose, Food analysis, Biosensor, Fructose dehydrogenase, Osmium redox, polymer
- in
- Food Chemistry
- volume
- 140
- issue
- 4
- pages
- 742 - 747
- publisher
- Elsevier
- external identifiers
-
- wos:000321083800019
- scopus:84878017647
- pmid:23692761
- ISSN
- 1873-7072
- DOI
- 10.1016/j.foodchem.2012.11.023
- language
- English
- LU publication?
- yes
- id
- b34614b2-9af0-4c37-891e-fc8dc0919455 (old id 3975344)
- date added to LUP
- 2016-04-01 14:22:04
- date last changed
- 2022-01-28 00:13:14
@article{b34614b2-9af0-4c37-891e-fc8dc0919455,
abstract = {{This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A cm(-2) mM), good reproducibility (RSD = 2.1%) and a fast response time (4 s). Finally, the biosensor was applied for specific determination of fructose in honey, fruit juices, soft and energy drinks. The results indicated a very good agreement with those obtained with a commercial reference kit. No significant interference was observed with the proposed biosensor. (C) 2012 Elsevier Ltd. All rights reserved.}},
author = {{Antiochia, Riccarda and Vinci, Giuliana and Gorton, Lo}},
issn = {{1873-7072}},
keywords = {{Fructose; Food analysis; Biosensor; Fructose dehydrogenase; Osmium redox; polymer}},
language = {{eng}},
number = {{4}},
pages = {{742--747}},
publisher = {{Elsevier}},
series = {{Food Chemistry}},
title = {{Rapid and direct determination of fructose in food: A new osmium-polymer mediated biosensor}},
url = {{http://dx.doi.org/10.1016/j.foodchem.2012.11.023}},
doi = {{10.1016/j.foodchem.2012.11.023}},
volume = {{140}},
year = {{2013}},
}