Miniaturization of multiplexed planar recombinant antibody arrays for serum protein profiling.
(2014) In Bioanalysis 6(9). p.1175-1185- Abstract
- Background: Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed. Results: In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm(2)/spot) and the array density was increased 19-times (38,000 spots/cm(2)). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen... (More)
- Background: Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed. Results: In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm(2)/spot) and the array density was increased 19-times (38,000 spots/cm(2)). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen nanolithography technology, and interfaced with a high-resolution fluorescent-based scanner. The reproducibility, sensitivity, specificity, and applicability of the set-up were demonstrated by profiling a set of well-characterized serum samples. Conclusion: The designed antibody array platform opens up new possibilities for large-scale, multiplex profiling of crude proteomes in a miniaturized fashion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4527947
- author
- Petersson, Linn ; Coen, Michael ; Amro, Nabil A ; Truedsson, Lennart LU ; Borrebaeck, Carl Ak and Wingren, Christer
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioanalysis
- volume
- 6
- issue
- 9
- pages
- 1175 - 1185
- publisher
- Future Science
- external identifiers
-
- pmid:24946919
- wos:000337945200012
- scopus:84903121058
- pmid:24946919
- ISSN
- 1757-6180
- DOI
- 10.4155/bio.13.342
- language
- English
- LU publication?
- yes
- id
- bc3f96e5-1b27-4814-84f0-e441e4f23691 (old id 4527947)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24946919?dopt=Abstract
- date added to LUP
- 2016-04-01 09:59:43
- date last changed
- 2022-03-27 03:54:46
@article{bc3f96e5-1b27-4814-84f0-e441e4f23691,
abstract = {{Background: Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed. Results: In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm(2)/spot) and the array density was increased 19-times (38,000 spots/cm(2)). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen nanolithography technology, and interfaced with a high-resolution fluorescent-based scanner. The reproducibility, sensitivity, specificity, and applicability of the set-up were demonstrated by profiling a set of well-characterized serum samples. Conclusion: The designed antibody array platform opens up new possibilities for large-scale, multiplex profiling of crude proteomes in a miniaturized fashion.}},
author = {{Petersson, Linn and Coen, Michael and Amro, Nabil A and Truedsson, Lennart and Borrebaeck, Carl Ak and Wingren, Christer}},
issn = {{1757-6180}},
language = {{eng}},
number = {{9}},
pages = {{1175--1185}},
publisher = {{Future Science}},
series = {{Bioanalysis}},
title = {{Miniaturization of multiplexed planar recombinant antibody arrays for serum protein profiling.}},
url = {{https://lup.lub.lu.se/search/files/1461749/5268135}},
doi = {{10.4155/bio.13.342}},
volume = {{6}},
year = {{2014}},
}